3uyi: Difference between revisions
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{{STRUCTURE_3uyi| PDB=3uyi | SCENE= }} | {{STRUCTURE_3uyi| PDB=3uyi | SCENE= }} | ||
===Crystal Structure of Perakine Reductase, Founder Member of a Novel AKR Subfamily with Unique Conformational Changes during NADPH Binding=== | ===Crystal Structure of Perakine Reductase, Founder Member of a Novel AKR Subfamily with Unique Conformational Changes during NADPH Binding=== | ||
{{ABSTRACT_PUBMED_22334702}} | |||
==Function== | |||
[[http://www.uniprot.org/uniprot/PERR_RAUSE PERR_RAUSE]] Aldo-keto reductase involved in the biosynthesis of monoterpenoid indole alkaloids. Broad substrate specificity enzyme with a high selectivity in the group of alkaloids. Can use perakine, 19(S),20(R)-dihydro-peraksine-17,21-al, cinnamic aldehyde, p-coumaric aldehyde and 3-(3,4,5-trimethoxyphenyl)propanal as substrates, but not ketosteroids such as progesterone. NADPH could not be replaced by NADH.<ref>PMID:18409028</ref> | |||
==About this Structure== | ==About this Structure== | ||
[[3uyi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rauvolfia_serpentina Rauvolfia serpentina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UYI OCA]. | [[3uyi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rauvolfia_serpentina Rauvolfia serpentina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UYI OCA]. | ||
==Reference== | |||
<ref group="xtra">PMID:022334702</ref><references group="xtra"/><references/> | |||
[[Category: Rauvolfia serpentina]] | [[Category: Rauvolfia serpentina]] | ||
[[Category: Chen, Y.]] | [[Category: Chen, Y.]] | ||
Revision as of 04:38, 18 July 2013
Crystal Structure of Perakine Reductase, Founder Member of a Novel AKR Subfamily with Unique Conformational Changes during NADPH Binding
Template:ABSTRACT PUBMED 22334702
Function
[PERR_RAUSE] Aldo-keto reductase involved in the biosynthesis of monoterpenoid indole alkaloids. Broad substrate specificity enzyme with a high selectivity in the group of alkaloids. Can use perakine, 19(S),20(R)-dihydro-peraksine-17,21-al, cinnamic aldehyde, p-coumaric aldehyde and 3-(3,4,5-trimethoxyphenyl)propanal as substrates, but not ketosteroids such as progesterone. NADPH could not be replaced by NADH.[1]
About this Structure
3uyi is a 1 chain structure with sequence from Rauvolfia serpentina. Full crystallographic information is available from OCA.
Reference
- Sun L, Chen Y, Rajendran C, Mueller U, Panjikar S, Wang M, Mindnich R, Rosenthal C, Penning TM, Stockigt J. Crystal structure of perakine reductase, founding member of a novel aldo-keto reductase (AKR) subfamily that undergoes unique conformational changes during NADPH binding. J Biol Chem. 2012 Mar 30;287(14):11213-21. Epub 2012 Feb 13. PMID:22334702 doi:10.1074/jbc.M111.335521
- ↑ Sun L, Ruppert M, Sheludko Y, Warzecha H, Zhao Y, Stockigt J. Purification, cloning, functional expression and characterization of perakine reductase: the first example from the AKR enzyme family, extending the alkaloidal network of the plant Rauvolfia. Plant Mol Biol. 2008 Jul;67(5):455-67. doi: 10.1007/s11103-008-9331-7. Epub 2008 , Apr 13. PMID:18409028 doi:10.1007/s11103-008-9331-7
