1x8b: Difference between revisions
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{{STRUCTURE_1x8b| PDB=1x8b | SCENE= }} | {{STRUCTURE_1x8b| PDB=1x8b | SCENE= }} | ||
===Structure of human Wee1A kinase: kinase domain complexed with inhibitor PD0407824=== | ===Structure of human Wee1A kinase: kinase domain complexed with inhibitor PD0407824=== | ||
{{ABSTRACT_PUBMED_15837193}} | |||
==Function== | |||
[[http://www.uniprot.org/uniprot/WEE1_HUMAN WEE1_HUMAN]] Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. | |||
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==About this Structure== | ==About this Structure== | ||
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==Reference== | ==Reference== | ||
<ref group="xtra">PMID:015837193</ref><references group="xtra"/> | <ref group="xtra">PMID:015837193</ref><references group="xtra"/><references/> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 08:25, 23 April 2014
Structure of human Wee1A kinase: kinase domain complexed with inhibitor PD0407824
Template:ABSTRACT PUBMED 15837193
Function
[WEE1_HUMAN] Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
About this Structure
1x8b is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Squire CJ, Dickson JM, Ivanovic I, Baker EN. Structure and inhibition of the human cell cycle checkpoint kinase, Wee1A kinase: an atypical tyrosine kinase with a key role in CDK1 regulation. Structure. 2005 Apr;13(4):541-50. PMID:15837193 doi:10.1016/j.str.2004.12.017
