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==Overview==

Topoisomerases I promote the relaxation of DNA superhelical tension by, introducing a transient single-stranded break in duplex DNA and are vital, for the processes of replication, transcription, and recombination. The, crystal structures at 2.1 and 2.5 angstrom resolution of reconstituted, human topoisomerase I comprising the core and carboxyl-terminal domains in, covalent and noncovalent complexes with 22-base pair DNA duplexes reveal, an enzyme that "clamps" around essentially B-form DNA. The core domain and, the first eight residues of the carboxyl-terminal domain of the enzyme, including the active-site nucleophile tyrosine-723, share significant, structural similarity with the bacteriophage family of DNA integrases. A, binding mode for the anticancer drug camptothecin is proposed on the basis, of chemical and biochemical information combined with these, three-dimensional structures of topoisomerase I-DNA complexes.

Topoisomerases I promote the relaxation of DNA superhelical tension by introducing a transient single-stranded break in duplex DNA and are vital for the processes of replication, transcription, and recombination. The crystal structures at 2.1 and 2.5 angstrom resolution of reconstituted human topoisomerase I comprising the core and carboxyl-terminal domains in covalent and noncovalent complexes with 22-base pair DNA duplexes reveal an enzyme that "clamps" around essentially B-form DNA. The core domain and the first eight residues of the carboxyl-terminal domain of the enzyme, including the active-site nucleophile tyrosine-723, share significant structural similarity with the bacteriophage family of DNA integrases. A binding mode for the anticancer drug camptothecin is proposed on the basis of chemical and biochemical information combined with these three-dimensional structures of topoisomerase I-DNA complexes.

==Disease==

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[[Category: Homo sapiens]]

[[Category: Single protein]]

[[Category: Champoux, J.J.]]

[[Category: Champoux, J J.]]

[[Category: Hol, W.G.]]

[[Category: Hol, W G.]]

[[Category: Kuhn, P.]]

[[Category: Redinbo, M.R.]]

[[Category: Redinbo, M R.]]

[[Category: Stewart, L.]]

[[Category: dna]]

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[[Category: topoisomerase i/dna)]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Topoisomerases I promote the relaxation of DNA superhelical tension by, introducing a transient single-stranded break in duplex DNA and are vital, for the processes of replication, transcription, and recombination. The, crystal structures at 2.1 and 2.5 angstrom resolution of reconstituted, human topoisomerase I comprising the core and carboxyl-terminal domains in, covalent and noncovalent complexes with 22-base pair DNA duplexes reveal, an enzyme that "clamps" around essentially B-form DNA. The core domain and, the first eight residues of the carboxyl-terminal domain of the enzyme, including the active-site nucleophile tyrosine-723, share significant, structural similarity with the bacteriophage family of DNA integrases. A, binding mode for the anticancer drug camptothecin is proposed on the basis, of chemical and biochemical information combined with these, three-dimensional structures of topoisomerase I-DNA complexes.
+Topoisomerases I promote the relaxation of DNA superhelical tension by introducing a transient single-stranded break in duplex DNA and are vital for the processes of replication, transcription, and recombination. The crystal structures at 2.1 and 2.5 angstrom resolution of reconstituted human topoisomerase I comprising the core and carboxyl-terminal domains in covalent and noncovalent complexes with 22-base pair DNA duplexes reveal an enzyme that "clamps" around essentially B-form DNA. The core domain and the first eight residues of the carboxyl-terminal domain of the enzyme, including the active-site nucleophile tyrosine-723, share significant structural similarity with the bacteriophage family of DNA integrases. A binding mode for the anticancer drug camptothecin is proposed on the basis of chemical and biochemical information combined with these three-dimensional structures of topoisomerase I-DNA complexes.
 ==Disease==
@@ Line 17: / Line 17: @@
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
-[[Category: Champoux, J.J.]]
+[[Category: Champoux, J J.]]
-[[Category: Hol, W.G.]]
+[[Category: Hol, W G.]]
 [[Category: Kuhn, P.]]
-[[Category: Redinbo, M.R.]]
+[[Category: Redinbo, M R.]]
 [[Category: Stewart, L.]]
 [[Category: dna]]
@@ Line 26: / Line 26: @@
 [[Category: topoisomerase i/dna)]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:28:16 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:40:17 2008''