Sandbox 35: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 58: | Line 58: | ||
==Other Interaction with Inhibitors and Effectors== | ==Other Interaction with Inhibitors and Effectors== | ||
[[Image:Papain Simple Cleavage.jpg|200px|right|thumb|Simple Overview of Papain | [[Image:Papain Simple Cleavage.jpg|200px|right|thumb|Simple Overview of Cleavage by Papain. <ref>[http://www.worthington-biochem.com/pap/default.html] Worthington Biochemical Corporation </ref>]] | ||
Except for valine, papain prefers to cleave at hydrophobic residues alanine, leucine, isoleucine, phenylalanine, tryptophan, or tyrosine <ref>[http://www.sigmaaldrich.com/life-science/biochemicals/biochemical-products.html?TablePage=16410606] Sigma Aldrich Papain</ref>. In light of the describe catalytic mechanism it makes sense that substances such as cysteine, sulfide/sulfite, heavy metal chelating agents like EDTA, and N-bromosuccinimide act as activators of the enzyme while PMSF, Hg2+ and other heavy metals, cystatin, leupeptin, sulfhydryl binding agents, carbonyl reagents, and alkylating agents serve as inhibitors. <ref>PMID: 6388564 </ref><ref>[http://www.biozym.de/datasheets/papain.php] Biozym </ref> | Except for valine, papain prefers to cleave at hydrophobic residues alanine, leucine, isoleucine, phenylalanine, tryptophan, or tyrosine <ref>[http://www.sigmaaldrich.com/life-science/biochemicals/biochemical-products.html?TablePage=16410606] Sigma Aldrich Papain</ref>. In light of the describe catalytic mechanism it makes sense that substances such as cysteine, sulfide/sulfite, heavy metal chelating agents like EDTA, and N-bromosuccinimide act as activators of the enzyme while PMSF, Hg2+ and other heavy metals, cystatin, leupeptin, sulfhydryl binding agents, carbonyl reagents, and alkylating agents serve as inhibitors. <ref>PMID: 6388564 </ref><ref>[http://www.biozym.de/datasheets/papain.php] Biozym </ref> | ||
| Line 64: | Line 64: | ||
==Fun Trivia== | ==Fun Trivia== | ||
Remember the 2002 SARS (Severe Acute Respiratory Syndrome) epidemic that placed global health, particularly in Southeast Asia, in a precarious state? On-going research is happening to further understand the mechanisms of this coronavirus, so that future steps can be taken for prevention. Its been found that the replication of RNA for this virus is mediated by two viral proteases that have many papain-like characteristics | Remember the 2002 SARS (Severe Acute Respiratory Syndrome) epidemic that placed global health, particularly in Southeast Asia, in a precarious state? On-going research is happening to further understand the mechanisms of this coronavirus, so that future steps can be taken for prevention. Its been found that the replication of RNA for this virus is mediated by two viral proteases that have many papain-like characteristics. <ref>PMID: 16306590 </ref> | ||
Despite a low percentage of sequence identities, inhibition and sequence analyses have increasingly been drawing parallels between L proteinases, that involve the foot-and-mouth disease virus and equine rhinovirus 1, and papain. With a similar overall fold to papain and identifiable regions that resemble the five alpha-helices and seven beta-sheets of papain, L proteinases of foot-and-mouth disease virus and of equine rhinovirus 1 reveal a mode of operation in the viruses that is very papain like. <ref>PMID: 9472614 </ref> | |||
==References== | ==References== | ||
