1d7q: Difference between revisions

Line 4:

==Overview==

The translation initiation factor eIF1A is necessary for directing the 43S, preinitiation complex from the 5' end of the mRNA to the initiation codon, in a process termed scanning. We have determined the solution structure of, human eIF1A, which reveals an oligonucleotide-binding (OB) fold and an, additional domain. NMR titration experiments showed that eIF1A binds, single-stranded RNA oligonucleotides in a site-specific, but, non-sequence-specific manner, hinting at an mRNA interaction rather than, specific rRNA or tRNA binding. The RNA binding surface extends over a, large area covering the canonical OB fold binding site as well as a groove, leading to the second domain. Site-directed mutations at multiple, positions along the RNA-binding surface were defective in the ability to, properly assemble preinitiation complexes at the AUG codon in vitro.

The translation initiation factor eIF1A is necessary for directing the 43S preinitiation complex from the 5' end of the mRNA to the initiation codon in a process termed scanning. We have determined the solution structure of human eIF1A, which reveals an oligonucleotide-binding (OB) fold and an additional domain. NMR titration experiments showed that eIF1A binds single-stranded RNA oligonucleotides in a site-specific, but non-sequence-specific manner, hinting at an mRNA interaction rather than specific rRNA or tRNA binding. The RNA binding surface extends over a large area covering the canonical OB fold binding site as well as a groove leading to the second domain. Site-directed mutations at multiple positions along the RNA-binding surface were defective in the ability to properly assemble preinitiation complexes at the AUG codon in vitro.

==About this Structure==

Line 13:

[[Category: Homo sapiens]]

[[Category: Single protein]]

[[Category: Battiste, J.L.]]

[[Category: Battiste, J L.]]

[[Category: Hellen, C.U.T.]]

[[Category: Hellen, C U.T.]]

[[Category: Pestova, T.V.]]

[[Category: Pestova, T V.]]

[[Category: Wagner, G.]]

[[Category: beta-barrel]]

Line 21:

[[Category: rna-binding protein]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri~~ Feb ~~15 15~~:38:55 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:13:51 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 4: / Line 4: @@
 ==Overview==
-The translation initiation factor eIF1A is necessary for directing the 43S, preinitiation complex from the 5' end of the mRNA to the initiation codon, in a process termed scanning. We have determined the solution structure of, human eIF1A, which reveals an oligonucleotide-binding (OB) fold and an, additional domain. NMR titration experiments showed that eIF1A binds, single-stranded RNA oligonucleotides in a site-specific, but, non-sequence-specific manner, hinting at an mRNA interaction rather than, specific rRNA or tRNA binding. The RNA binding surface extends over a, large area covering the canonical OB fold binding site as well as a groove, leading to the second domain. Site-directed mutations at multiple, positions along the RNA-binding surface were defective in the ability to, properly assemble preinitiation complexes at the AUG codon in vitro.
+The translation initiation factor eIF1A is necessary for directing the 43S preinitiation complex from the 5' end of the mRNA to the initiation codon in a process termed scanning. We have determined the solution structure of human eIF1A, which reveals an oligonucleotide-binding (OB) fold and an additional domain. NMR titration experiments showed that eIF1A binds single-stranded RNA oligonucleotides in a site-specific, but non-sequence-specific manner, hinting at an mRNA interaction rather than specific rRNA or tRNA binding. The RNA binding surface extends over a large area covering the canonical OB fold binding site as well as a groove leading to the second domain. Site-directed mutations at multiple positions along the RNA-binding surface were defective in the ability to properly assemble preinitiation complexes at the AUG codon in vitro.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
-[[Category: Battiste, J.L.]]
+[[Category: Battiste, J L.]]
-[[Category: Hellen, C.U.T.]]
+[[Category: Hellen, C U.T.]]
-[[Category: Pestova, T.V.]]
+[[Category: Pestova, T V.]]
 [[Category: Wagner, G.]]
 [[Category: beta-barrel]]
@@ Line 21: / Line 21: @@
 [[Category: rna-binding protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:38:55 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:13:51 2008''