1deg: Difference between revisions

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==Overview==

The crystal structure of a mutant calmodulin (CaM) lacking Glu-84 has been, refined to R = 0.23 using data measured to 2.9-A resolution. In native CaM, the central helix is fully extended, and the molecule is dumbbell shaped., In contrast, the deletion of Glu-84 causes a bend of 95 degrees in the, linker region of the central helix at Ile-85. However, EF-hand domains 1, and 2 (lobe 1,2) do not touch lobe 3,4. The length, by alpha-carbon, separation, of des-Glu84-CaM is 56 A; that of native CaM is 64 A. The, shape of des-Glu84-CaM is similar to that of native CaM, as it is bound to, the target peptide of myosin light-chain kinase. This result supports the, proposal that the linker region of the central helix of CaM functions as a, flexible tether.

The crystal structure of a mutant calmodulin (CaM) lacking Glu-84 has been refined to R = 0.23 using data measured to 2.9-A resolution. In native CaM the central helix is fully extended, and the molecule is dumbbell shaped. In contrast, the deletion of Glu-84 causes a bend of 95 degrees in the linker region of the central helix at Ile-85. However, EF-hand domains 1 and 2 (lobe 1,2) do not touch lobe 3,4. The length, by alpha-carbon separation, of des-Glu84-CaM is 56 A; that of native CaM is 64 A. The shape of des-Glu84-CaM is similar to that of native CaM, as it is bound to the target peptide of myosin light-chain kinase. This result supports the proposal that the linker region of the central helix of CaM functions as a flexible tether.

==Disease==

Line 17:

[[Category: Single protein]]

[[Category: Chandross, R.]]

[[Category: Cheng, B.P.]]

[[Category: Cheng, B P.]]

[[Category: Kretsinger, R.H.]]

[[Category: Kretsinger, R H.]]

[[Category: Persechini, A.]]

[[Category: Raghunathan, S.]]

[[Category: Sobottk, S.E.]]

[[Category: Sobottk, S E.]]

[[Category: CA]]

[[Category: calcium-binding protein]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-The crystal structure of a mutant calmodulin (CaM) lacking Glu-84 has been, refined to R = 0.23 using data measured to 2.9-A resolution. In native CaM, the central helix is fully extended, and the molecule is dumbbell shaped., In contrast, the deletion of Glu-84 causes a bend of 95 degrees in the, linker region of the central helix at Ile-85. However, EF-hand domains 1, and 2 (lobe 1,2) do not touch lobe 3,4. The length, by alpha-carbon, separation, of des-Glu84-CaM is 56 A; that of native CaM is 64 A. The, shape of des-Glu84-CaM is similar to that of native CaM, as it is bound to, the target peptide of myosin light-chain kinase. This result supports the, proposal that the linker region of the central helix of CaM functions as a, flexible tether.
+The crystal structure of a mutant calmodulin (CaM) lacking Glu-84 has been refined to R = 0.23 using data measured to 2.9-A resolution. In native CaM the central helix is fully extended, and the molecule is dumbbell shaped. In contrast, the deletion of Glu-84 causes a bend of 95 degrees in the linker region of the central helix at Ile-85. However, EF-hand domains 1 and 2 (lobe 1,2) do not touch lobe 3,4. The length, by alpha-carbon separation, of des-Glu84-CaM is 56 A; that of native CaM is 64 A. The shape of des-Glu84-CaM is similar to that of native CaM, as it is bound to the target peptide of myosin light-chain kinase. This result supports the proposal that the linker region of the central helix of CaM functions as a flexible tether.
 ==Disease==
@@ Line 17: / Line 17: @@
 [[Category: Single protein]]
 [[Category: Chandross, R.]]
-[[Category: Cheng, B.P.]]
+[[Category: Cheng, B P.]]
-[[Category: Kretsinger, R.H.]]
+[[Category: Kretsinger, R H.]]
 [[Category: Persechini, A.]]
 [[Category: Raghunathan, S.]]
-[[Category: Sobottk, S.E.]]
+[[Category: Sobottk, S E.]]
 [[Category: CA]]
 [[Category: calcium-binding protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:39:26 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:15:49 2008''