Sandbox 49: Difference between revisions
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==Inhibition and Activation== | ==Inhibition and Activation== | ||
C11 alkyl phosphate is an effective inhibitor of pancreatic lipase, it was synthesized in an attempt to gain a better understanding of the enzyme’s active site. The inhibitor’s alkyl chain fits into the hydrophobic groove of the active site’s catalytic triad and covalently binds to Ser 152, but instead of reacting like the normal triacylglyceride substrate the inhibitor does not react and stays bound, effectively destroying the ability of the enzyme to function. | |||
Bile salts are steroids important for further degradation of the triacylglycerides processed by pancreatic lipase, however they are also inhibitors of the lipase as well. To prevent this inhibition the cofactor colipase is vital. Colipase is secreted by the pancreas in its inactive form procolipase, upon activation the protein binds to the noncatalytic C terminal arm of Lipase to activate the protein. | |||
==References== | ==References== | ||
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*http://pubs.acs.org/doi/abs/10.1021/bi00009a003 | *http://pubs.acs.org/doi/abs/10.1021/bi00009a003 | ||
*http://www.ncbi.nlm.nih.gov/pubmed/7929247 | *http://www.ncbi.nlm.nih.gov/pubmed/7929247 | ||
* | *http://www.ebi.ac.uk/interpro/IEntry?ac=IPR001981 | ||
*http://www.ncbi.nlm.nih.gov/pubmed/500812 | |||
