2y95: Difference between revisions

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[[~~Image~~:2y95.~~png|left|200px~~]]

==Solution structure of AUCG tetraloop hairpin found in human Xist RNA A-repeats essential for X-inactivation==

== Structural highlights ==

<table><tr><td colspan='2'>[[2y95]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y95 OCA]. <br>

</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>

<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y95 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2y95 RCSB], [http://www.ebi.ac.uk/pdbsum/2y95 PDBsum]</span></td></tr>

<table>

== Publication Abstract from PubMed ==

X-chromosome inactivation (XCI) in female mammals depends on the noncoding RNA X inactivation specific transcript (Xist). The mechanism of chromosome-wide silencing by Xist is poorly understood. While it is established that the 5' region of Xist RNA, comprising the A-repeats and holding 7.5-8.5 copies of a conserved 26-mer sequence, is essential for Xist-mediated silencing, high-resolution structural information for the A-repeats is not available. Here, we report the three-dimensional solution structure of a 14-mer hairpin in the 5' region of a human A-repeat. This hairpin is remarkably stable and adopts a novel AUCG tetraloop fold, the integrity of which is required for silencing. We show that, contrary to previous predictions, the 3' region of single or tandem A-repeats mediates duplex formation in vitro. Significantly, mutations in this region disrupt the inter-repeat duplex formation in vitro and abrogate the silencing function of Xist A-repeats in vivo. Our data suggest that the complete A-repeat region may be stabilized by inter-repeat duplex formation and, as such, may provide a platform for multimerization and specific recognition of the AUCG tetraloops by trans-acting factors.

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The Xist RNA A-repeat comprises a novel AUCG tetraloop fold and a platform for multimerization.,Duszczyk MM, Wutz A, Rybin V, Sattler M RNA. 2011 Nov;17(11):1973-82. Epub 2011 Sep 26. PMID:21947263<ref>PMID:21947263</ref>

~~The line below this paragraph~~, ~~containing "STRUCTURE_2y95"~~, ~~creates the "Structure Box" on the page~~.

~~You may change the PDB parameter~~ (~~which sets the PDB file loaded into the applet~~)

~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~

~~or leave the SCENE parameter empty for the default display~~.

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~~{{STRUCTURE_2y95| PDB=2y95 | SCENE= }}~~

~~===Solution structure~~ of ~~AUCG tetraloop hairpin found in human Xist RNA A-repeats essential for X-inactivation===~~

From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>

</div>

== References ==

~~<!--~~

~~The line below this paragraph, {{ABSTRACT_PUBMED_21947263}}, adds the Publication Abstract to~~ the ~~page~~

__TOC__

~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 21947263 is the PubMed ID number~~.

</StructureSection>

-->

~~{{ABSTRACT_PUBMED_21947263}}~~

~~==About this Structure==~~

~~[[2y95]] is a 1 chain structure. Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=2Y95 OCA].~~

==~~Reference~~==

<~~ref group="xtra">PMID:021947263<~~/~~ref~~><~~ref group="xtra">PMID:021779925</ref><references group="xtra"~~/>

[[Category: Duszczyk, M M.]]

[[Category: Sattler, M.]]

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@@ Line 1: / Line 1: @@
-[[Image:2y95.png|left|200px]]
+==Solution structure of AUCG tetraloop hairpin found in human Xist RNA A-repeats essential for X-inactivation==
+<StructureSection load='2y95' size='340' side='right' caption='[[2y95]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2y95]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y95 OCA]. <br>
+</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2y95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y95 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2y95 RCSB], [http://www.ebi.ac.uk/pdbsum/2y95 PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+X-chromosome inactivation (XCI) in female mammals depends on the noncoding RNA X inactivation specific transcript (Xist). The mechanism of chromosome-wide silencing by Xist is poorly understood. While it is established that the 5' region of Xist RNA, comprising the A-repeats and holding 7.5-8.5 copies of a conserved 26-mer sequence, is essential for Xist-mediated silencing, high-resolution structural information for the A-repeats is not available. Here, we report the three-dimensional solution structure of a 14-mer hairpin in the 5' region of a human A-repeat. This hairpin is remarkably stable and adopts a novel AUCG tetraloop fold, the integrity of which is required for silencing. We show that, contrary to previous predictions, the 3' region of single or tandem A-repeats mediates duplex formation in vitro. Significantly, mutations in this region disrupt the inter-repeat duplex formation in vitro and abrogate the silencing function of Xist A-repeats in vivo. Our data suggest that the complete A-repeat region may be stabilized by inter-repeat duplex formation and, as such, may provide a platform for multimerization and specific recognition of the AUCG tetraloops by trans-acting factors.
-<!--
+The Xist RNA A-repeat comprises a novel AUCG tetraloop fold and a platform for multimerization.,Duszczyk MM, Wutz A, Rybin V, Sattler M RNA. 2011 Nov;17(11):1973-82. Epub 2011 Sep 26. PMID:21947263<ref>PMID:21947263</ref>
-The line below this paragraph, containing "STRUCTURE_2y95", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
--->
-{{STRUCTURE_2y95|  PDB=2y95  |  SCENE=  }}
-===Solution structure of AUCG tetraloop hairpin found in human Xist RNA A-repeats essential for X-inactivation===
+From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+</div>
+== References ==
-<!--
+<references/>
-The line below this paragraph, {{ABSTRACT_PUBMED_21947263}}, adds the Publication Abstract to the page
+__TOC__
-(as it appears on PubMed at http://www.pubmed.gov), where 21947263 is the PubMed ID number.
+</StructureSection>
--->
-{{ABSTRACT_PUBMED_21947263}}
-==About this Structure==
-[[2y95]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y95 OCA].
-==Reference==
-<ref group="xtra">PMID:021947263</ref><ref group="xtra">PMID:021779925</ref><references group="xtra"/>
 [[Category: Duszczyk, M M.]]
 [[Category: Sattler, M.]]