1f4y: Difference between revisions

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==Overview==

The crystal structure of the murine Fab S-20-4 from a protective, anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa, serotype has been determined in its unliganded form and in complex with, synthetic fragments of the Ogawa O-specific polysaccharide (O-SP). The, upstream terminal O-SP monosaccharide is shown to be the primary antigenic, determinant. Additional perosamine residues protrude outwards from the Ab, surface and contribute only marginally to the binding affinity and, specificity. A complementary water-excluding hydrophobic interface and, five Ab-Ag hydrogen bonds are crucial for carbohydrate recognition. The, structure reported here explains the serotype specificity of anti-Ogawa, Abs and provides a rational basis toward the development of a synthetic, carbohydrate-based anti-cholera vaccine.

The crystal structure of the murine Fab S-20-4 from a protective anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa serotype has been determined in its unliganded form and in complex with synthetic fragments of the Ogawa O-specific polysaccharide (O-SP). The upstream terminal O-SP monosaccharide is shown to be the primary antigenic determinant. Additional perosamine residues protrude outwards from the Ab surface and contribute only marginally to the binding affinity and specificity. A complementary water-excluding hydrophobic interface and five Ab-Ag hydrogen bonds are crucial for carbohydrate recognition. The structure reported here explains the serotype specificity of anti-Ogawa Abs and provides a rational basis toward the development of a synthetic carbohydrate-based anti-cholera vaccine.

==About this Structure==

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[[Category: Mus musculus]]

[[Category: Protein complex]]

[[Category: Alzari, P.M.]]

[[Category: Alzari, P M.]]

[[Category: Souchon, H.]]

[[Category: MGU]]

[[Category: anti-carbohydrate antibody]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri~~ Feb ~~15 15~~:45:54 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:34:54 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The crystal structure of the murine Fab S-20-4 from a protective, anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa, serotype has been determined in its unliganded form and in complex with, synthetic fragments of the Ogawa O-specific polysaccharide (O-SP). The, upstream terminal O-SP monosaccharide is shown to be the primary antigenic, determinant. Additional perosamine residues protrude outwards from the Ab, surface and contribute only marginally to the binding affinity and, specificity. A complementary water-excluding hydrophobic interface and, five Ab-Ag hydrogen bonds are crucial for carbohydrate recognition. The, structure reported here explains the serotype specificity of anti-Ogawa, Abs and provides a rational basis toward the development of a synthetic, carbohydrate-based anti-cholera vaccine.
+The crystal structure of the murine Fab S-20-4 from a protective anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa serotype has been determined in its unliganded form and in complex with synthetic fragments of the Ogawa O-specific polysaccharide (O-SP). The upstream terminal O-SP monosaccharide is shown to be the primary antigenic determinant. Additional perosamine residues protrude outwards from the Ab surface and contribute only marginally to the binding affinity and specificity. A complementary water-excluding hydrophobic interface and five Ab-Ag hydrogen bonds are crucial for carbohydrate recognition. The structure reported here explains the serotype specificity of anti-Ogawa Abs and provides a rational basis toward the development of a synthetic carbohydrate-based anti-cholera vaccine.
 ==About this Structure==
@@ Line 13: / Line 13: @@
 [[Category: Mus musculus]]
 [[Category: Protein complex]]
-[[Category: Alzari, P.M.]]
+[[Category: Alzari, P M.]]
 [[Category: Souchon, H.]]
 [[Category: MGU]]
 [[Category: anti-carbohydrate antibody]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:45:54 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:34:54 2008''