1h3p: Difference between revisions
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==Overview== | ==Overview== | ||
The monoclonal antibody 5a19, raised against the ay serotype of hepatitis | The monoclonal antibody 5a19, raised against the ay serotype of hepatitis B virus, binds to the segment of the preS1 region comprising residues 37-43, which is implicated in attachment of the virus to hepatocytes. The dissociation constant, derived from kinetic studies using surface plasmon resonance techniques, is in the low nanomolar range. The nucleotide sequence of the variable domains has been determined and the corresponding germ-line genes have been identified. The three-dimensional structure of the Fab fragment has been determined by X-ray crystallography to 2.6 A resolution. | ||
==About this Structure== | ==About this Structure== | ||
1H3P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure | 1H3P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. This structure supersedes the now removed PDB entry 1GM3. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H3P OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bentley, G | [[Category: Bentley, G A.]] | ||
[[Category: Budkowska, A.]] | [[Category: Budkowska, A.]] | ||
[[Category: Pizarro, J | [[Category: Pizarro, J C.]] | ||
[[Category: Riottot, M | [[Category: Riottot, M M.]] | ||
[[Category: Vulliez-Le-Normand, B.]] | [[Category: Vulliez-Le-Normand, B.]] | ||
[[Category: antibody fab fragment]] | [[Category: antibody fab fragment]] | ||
[[Category: immunoglobulin]] | [[Category: immunoglobulin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:57:05 2008'' | ||
Revision as of 10:57, 21 February 2008
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STRUCTURAL CHARACTERISATION OF A MONOCLONAL ANTIBODY SPECIFIC FOR THE PRES1 REGION OF THE HEPATITIS B VIRUS
Overview
The monoclonal antibody 5a19, raised against the ay serotype of hepatitis B virus, binds to the segment of the preS1 region comprising residues 37-43, which is implicated in attachment of the virus to hepatocytes. The dissociation constant, derived from kinetic studies using surface plasmon resonance techniques, is in the low nanomolar range. The nucleotide sequence of the variable domains has been determined and the corresponding germ-line genes have been identified. The three-dimensional structure of the Fab fragment has been determined by X-ray crystallography to 2.6 A resolution.
About this Structure
1H3P is a Single protein structure of sequence from Mus musculus. This structure supersedes the now removed PDB entry 1GM3. Full crystallographic information is available from OCA.
Reference
Structural and functional characterization of a monoclonal antibody specific for the preS1 region of hepatitis B virus., Pizarro JC, Vulliez-le Normand B, Riottot MM, Budkowska A, Bentley GA, FEBS Lett. 2001 Dec 14;509(3):463-8. PMID:11749974
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