1i7z: Difference between revisions

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==Overview==

Murine monoclonal antibody GNC92H2 was elicited by active immunization, with a cocaine immunoconjugate and binds free cocaine with excellent, specificity and moderate affinity. Improvement of affinity, as well as, humanization of GNC92H2, would be advantageous in immunopharmacotherapy, for cocaine addiction, and for emergency cases of drug overdose. Toward, this end, the crystal structure of an engineered murine-human chimeric Fab, of GNC92H2 complexed with cocaine was determined at 2.3 A resolution., Structural analysis reveals a binding pocket with high shape and charge, complementarity to the cocaine framework, which explains the specificity, for cocaine, as opposed to the pharmacologically inactive cocaine, metabolites. Importantly, the structure provides a foundation for, mutagenesis to enhance the binding affinity for cocaine and potent cocaine, derivatives, such as cocaethylene, and for additional humanization of the, antibody.

Murine monoclonal antibody GNC92H2 was elicited by active immunization with a cocaine immunoconjugate and binds free cocaine with excellent specificity and moderate affinity. Improvement of affinity, as well as humanization of GNC92H2, would be advantageous in immunopharmacotherapy for cocaine addiction, and for emergency cases of drug overdose. Toward this end, the crystal structure of an engineered murine-human chimeric Fab of GNC92H2 complexed with cocaine was determined at 2.3 A resolution. Structural analysis reveals a binding pocket with high shape and charge complementarity to the cocaine framework, which explains the specificity for cocaine, as opposed to the pharmacologically inactive cocaine metabolites. Importantly, the structure provides a foundation for mutagenesis to enhance the binding affinity for cocaine and potent cocaine derivatives, such as cocaethylene, and for additional humanization of the antibody.

==Disease==

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[[Category: Mus musculus and homo sapiens]]

[[Category: Protein complex]]

[[Category: Larsen, N.A.]]

[[Category: Larsen, N A.]]

[[Category: Wilson, I.A.]]

[[Category: Wilson, I A.]]

[[Category: COC]]

[[Category: antibody]]

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[[Category: igg fold]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:09:02 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-Murine monoclonal antibody GNC92H2 was elicited by active immunization, with a cocaine immunoconjugate and binds free cocaine with excellent, specificity and moderate affinity. Improvement of affinity, as well as, humanization of GNC92H2, would be advantageous in immunopharmacotherapy, for cocaine addiction, and for emergency cases of drug overdose. Toward, this end, the crystal structure of an engineered murine-human chimeric Fab, of GNC92H2 complexed with cocaine was determined at 2.3 A resolution., Structural analysis reveals a binding pocket with high shape and charge, complementarity to the cocaine framework, which explains the specificity, for cocaine, as opposed to the pharmacologically inactive cocaine, metabolites. Importantly, the structure provides a foundation for, mutagenesis to enhance the binding affinity for cocaine and potent cocaine, derivatives, such as cocaethylene, and for additional humanization of the, antibody.
+Murine monoclonal antibody GNC92H2 was elicited by active immunization with a cocaine immunoconjugate and binds free cocaine with excellent specificity and moderate affinity. Improvement of affinity, as well as humanization of GNC92H2, would be advantageous in immunopharmacotherapy for cocaine addiction, and for emergency cases of drug overdose. Toward this end, the crystal structure of an engineered murine-human chimeric Fab of GNC92H2 complexed with cocaine was determined at 2.3 A resolution. Structural analysis reveals a binding pocket with high shape and charge complementarity to the cocaine framework, which explains the specificity for cocaine, as opposed to the pharmacologically inactive cocaine metabolites. Importantly, the structure provides a foundation for mutagenesis to enhance the binding affinity for cocaine and potent cocaine derivatives, such as cocaethylene, and for additional humanization of the antibody.
 ==Disease==
@@ Line 16: / Line 16: @@
 [[Category: Mus musculus and homo sapiens]]
 [[Category: Protein complex]]
-[[Category: Larsen, N.A.]]
+[[Category: Larsen, N A.]]
-[[Category: Wilson, I.A.]]
+[[Category: Wilson, I A.]]
 [[Category: COC]]
 [[Category: antibody]]
@@ Line 23: / Line 23: @@
 [[Category: igg fold]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 15:59:53 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:09:02 2008''