Lipase: Difference between revisions
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[[Image:M0218.stg01.gif]] | [[Image:M0218.stg01.gif]] | ||
The carbonyl reforms with the glycerol backbone segment acting as the leaving group (Reaction 2). | The carbonyl reforms with the glycerol backbone segment acting as the leaving group (Reaction 2). | ||
[[Image:M0218.stg02.gif]] | [[Image:M0218.stg02.gif]] | ||
A water molecule then donates a proton to the histidine, creating a reactive hydroxyl anion, which can attack the carbonyl carbon of the lipid, forming another negatively charged tetrahedral intermediate which is stabilized in the oxyanion hole (Reaction 3). | A water molecule then donates a proton to the histidine, creating a reactive hydroxyl anion, which can attack the carbonyl carbon of the lipid, forming another negatively charged tetrahedral intermediate which is stabilized in the oxyanion hole (Reaction 3). | ||
[[Image:M0218.stg03.gif]] | [[Image:M0218.stg03.gif]] | ||
Upon reformation of the carbonyl, the catalytic serine is released and monoglyceride and fatty acid monomers diffuse away (Reaction 4). | Upon reformation of the carbonyl, the catalytic serine is released and monoglyceride and fatty acid monomers diffuse away (Reaction 4). | ||
[[Image:M0218.stg04.gif]] | [[Image:M0218.stg04.gif]] | ||
