1l0i: Difference between revisions

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==Overview==

Acyl carrier protein (ACP) is an essential cofactor in biosynthesis of, fatty acids and many other reactions that require acyl transfer steps. We, have determined the first crystal structures of an acylated form of ACP, from E. coli, that of butyryl-ACP. Our analysis of the molecular surface, of ACP reveals a plastic hydrophobic cavity in the vicinity of the, phosphopantethylated Ser36 residue that is expanded and occupied by the, butyryl and beta-mercaptoethylamine moieties of the acylated, 4'-phosphopantetheine group in one of our crystal forms. In the other, form, the cavity is contracted, and we propose that the protein has, adopted the conformation after delivery of substrate into the active site, of a partner enzyme.

Acyl carrier protein (ACP) is an essential cofactor in biosynthesis of fatty acids and many other reactions that require acyl transfer steps. We have determined the first crystal structures of an acylated form of ACP from E. coli, that of butyryl-ACP. Our analysis of the molecular surface of ACP reveals a plastic hydrophobic cavity in the vicinity of the phosphopantethylated Ser36 residue that is expanded and occupied by the butyryl and beta-mercaptoethylamine moieties of the acylated 4'-phosphopantetheine group in one of our crystal forms. In the other form, the cavity is contracted, and we propose that the protein has adopted the conformation after delivery of substrate into the active site of a partner enzyme.

==About this Structure==

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[[Category: Fatty Acid Synthase]]

[[Category: Single protein]]

[[Category: Baker, P.J.]]

[[Category: Baker, P J.]]

[[Category: Baldock, C.]]

[[Category: Gilroy, J.]]

[[Category: Rafferty, J.B.]]

[[Category: Rafferty, J B.]]

[[Category: Rice, D.W.]]

[[Category: Rice, D W.]]

[[Category: Roujeinikova, A.]]

[[Category: Simon, W.J.]]

[[Category: Simon, W J.]]

[[Category: Slabas, A.R.]]

[[Category: Slabas, A R.]]

[[Category: Stuitje, A.R.]]

[[Category: Stuitje, A R.]]

[[Category: CAC]]

[[Category: NA]]

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[[Category: fatty acid biosynthesis]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Acyl carrier protein (ACP) is an essential cofactor in biosynthesis of, fatty acids and many other reactions that require acyl transfer steps. We, have determined the first crystal structures of an acylated form of ACP, from E. coli, that of butyryl-ACP. Our analysis of the molecular surface, of ACP reveals a plastic hydrophobic cavity in the vicinity of the, phosphopantethylated Ser36 residue that is expanded and occupied by the, butyryl and beta-mercaptoethylamine moieties of the acylated, 4'-phosphopantetheine group in one of our crystal forms. In the other, form, the cavity is contracted, and we propose that the protein has, adopted the conformation after delivery of substrate into the active site, of a partner enzyme.
+Acyl carrier protein (ACP) is an essential cofactor in biosynthesis of fatty acids and many other reactions that require acyl transfer steps. We have determined the first crystal structures of an acylated form of ACP from E. coli, that of butyryl-ACP. Our analysis of the molecular surface of ACP reveals a plastic hydrophobic cavity in the vicinity of the phosphopantethylated Ser36 residue that is expanded and occupied by the butyryl and beta-mercaptoethylamine moieties of the acylated 4'-phosphopantetheine group in one of our crystal forms. In the other form, the cavity is contracted, and we propose that the protein has adopted the conformation after delivery of substrate into the active site of a partner enzyme.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Fatty Acid Synthase]]
 [[Category: Single protein]]
-[[Category: Baker, P.J.]]
+[[Category: Baker, P J.]]
 [[Category: Baldock, C.]]
 [[Category: Gilroy, J.]]
-[[Category: Rafferty, J.B.]]
+[[Category: Rafferty, J B.]]
-[[Category: Rice, D.W.]]
+[[Category: Rice, D W.]]
 [[Category: Roujeinikova, A.]]
-[[Category: Simon, W.J.]]
+[[Category: Simon, W J.]]
-[[Category: Slabas, A.R.]]
+[[Category: Slabas, A R.]]
-[[Category: Stuitje, A.R.]]
+[[Category: Stuitje, A R.]]
 [[Category: CAC]]
 [[Category: NA]]
@@ Line 32: / Line 32: @@
 [[Category: fatty acid biosynthesis]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:16:09 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:39:57 2008''