1lv1: Difference between revisions

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==Overview==

Autolysis rates of the C95M and C95M/C1095A mutants of a HIV-1 protease, tethered dimer have been determined by real time NMR and it is observed, that the double mutant has approximately two times higher rate. X-ray, structure of the C95M/C1095A double mutant has been solved and refined to, 2.1 A resolution. Comparison of the double mutant structure with that of, C95M single mutant reveals that there is a shift in the position of the, catalytic aspartates and the bound catalytic water. The mutation also, causes a loss of hydrophobic packing near the dimerization domain of the, protein. These observations demonstrate that subtle changes are adequate, to cause significant changes in the rate of autolysis of the double, mutant. This provides a rationale for the effects of remote mutations on, the activity and drug resistance of the enzyme.

Autolysis rates of the C95M and C95M/C1095A mutants of a HIV-1 protease tethered dimer have been determined by real time NMR and it is observed that the double mutant has approximately two times higher rate. X-ray structure of the C95M/C1095A double mutant has been solved and refined to 2.1 A resolution. Comparison of the double mutant structure with that of C95M single mutant reveals that there is a shift in the position of the catalytic aspartates and the bound catalytic water. The mutation also causes a loss of hydrophobic packing near the dimerization domain of the protein. These observations demonstrate that subtle changes are adequate to cause significant changes in the rate of autolysis of the double mutant. This provides a rationale for the effects of remote mutations on the activity and drug resistance of the enzyme.

==About this Structure==

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[[Category: Human immunodeficiency virus 1]]

[[Category: Single protein]]

[[Category: Bhavesh, N.S.]]

[[Category: Bhavesh, N S.]]

[[Category: Chatterjee, A.]]

[[Category: Hosur, M.V.]]

[[Category: Hosur, M V.]]

[[Category: Hosur, R.V.]]

[[Category: Hosur, R V.]]

[[Category: Kannan, K.K.]]

[[Category: Kannan, K K.]]

[[Category: Kumar, M.]]

[[Category: Mittal, R.]]

[[Category: beta-ribbon flap]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:47 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-Autolysis rates of the C95M and C95M/C1095A mutants of a HIV-1 protease, tethered dimer have been determined by real time NMR and it is observed, that the double mutant has approximately two times higher rate. X-ray, structure of the C95M/C1095A double mutant has been solved and refined to, 2.1 A resolution. Comparison of the double mutant structure with that of, C95M single mutant reveals that there is a shift in the position of the, catalytic aspartates and the bound catalytic water. The mutation also, causes a loss of hydrophobic packing near the dimerization domain of the, protein. These observations demonstrate that subtle changes are adequate, to cause significant changes in the rate of autolysis of the double, mutant. This provides a rationale for the effects of remote mutations on, the activity and drug resistance of the enzyme.
+Autolysis rates of the C95M and C95M/C1095A mutants of a HIV-1 protease tethered dimer have been determined by real time NMR and it is observed that the double mutant has approximately two times higher rate. X-ray structure of the C95M/C1095A double mutant has been solved and refined to 2.1 A resolution. Comparison of the double mutant structure with that of C95M single mutant reveals that there is a shift in the position of the catalytic aspartates and the bound catalytic water. The mutation also causes a loss of hydrophobic packing near the dimerization domain of the protein. These observations demonstrate that subtle changes are adequate to cause significant changes in the rate of autolysis of the double mutant. This provides a rationale for the effects of remote mutations on the activity and drug resistance of the enzyme.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Human immunodeficiency virus 1]]
 [[Category: Single protein]]
-[[Category: Bhavesh, N.S.]]
+[[Category: Bhavesh, N S.]]
 [[Category: Chatterjee, A.]]
-[[Category: Hosur, M.V.]]
+[[Category: Hosur, M V.]]
-[[Category: Hosur, R.V.]]
+[[Category: Hosur, R V.]]
-[[Category: Kannan, K.K.]]
+[[Category: Kannan, K K.]]
 [[Category: Kumar, M.]]
 [[Category: Mittal, R.]]
 [[Category: beta-ribbon flap]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:20:36 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:47 2008''