1m9f: Difference between revisions

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==Overview==

Cyclophilins constitute a ubiquitous protein family whose functions, include protein folding, transport and signaling. They possess both, sequence-specific binding and proline cis-trans isomerase activities, as, exemplified by the interaction between cyclophilin A (CypA) and the HIV-1, CA protein. Here, we report crystal structures of CypA in complex with, HIV-1 CA protein variants that bind preferentially with the substrate, proline residue in either the cis or the trans conformation. Cis- and, trans-Pro substrates are accommodated within the enzyme active site by, rearrangement of their N-terminal residues and with minimal distortions in, the path of the main chain. CypA Arg55 guanidinium group probably, facilitates catalysis by anchoring the substrate proline oxygen and, stabilizing sp3 hybridization of the proline nitrogen in the transition, state.

Cyclophilins constitute a ubiquitous protein family whose functions include protein folding, transport and signaling. They possess both sequence-specific binding and proline cis-trans isomerase activities, as exemplified by the interaction between cyclophilin A (CypA) and the HIV-1 CA protein. Here, we report crystal structures of CypA in complex with HIV-1 CA protein variants that bind preferentially with the substrate proline residue in either the cis or the trans conformation. Cis- and trans-Pro substrates are accommodated within the enzyme active site by rearrangement of their N-terminal residues and with minimal distortions in the path of the main chain. CypA Arg55 guanidinium group probably facilitates catalysis by anchoring the substrate proline oxygen and stabilizing sp3 hybridization of the proline nitrogen in the transition state.

==About this Structure==

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[[Category: Peptidylprolyl isomerase]]

[[Category: Protein complex]]

[[Category: Hill, C.P.]]

[[Category: Hill, C P.]]

[[Category: Howard, B.R.]]

[[Category: Howard, B R.]]

[[Category: Li, S.]]

[[Category: Sundquist, W.I.]]

[[Category: Sundquist, W I.]]

[[Category: Vajdos, F.F.]]

[[Category: Vajdos, F F.]]

[[Category: capsid]]

[[Category: cyclophilin a]]

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[[Category: rotamase]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Cyclophilins constitute a ubiquitous protein family whose functions, include protein folding, transport and signaling. They possess both, sequence-specific binding and proline cis-trans isomerase activities, as, exemplified by the interaction between cyclophilin A (CypA) and the HIV-1, CA protein. Here, we report crystal structures of CypA in complex with, HIV-1 CA protein variants that bind preferentially with the substrate, proline residue in either the cis or the trans conformation. Cis- and, trans-Pro substrates are accommodated within the enzyme active site by, rearrangement of their N-terminal residues and with minimal distortions in, the path of the main chain. CypA Arg55 guanidinium group probably, facilitates catalysis by anchoring the substrate proline oxygen and, stabilizing sp3 hybridization of the proline nitrogen in the transition, state.
+Cyclophilins constitute a ubiquitous protein family whose functions include protein folding, transport and signaling. They possess both sequence-specific binding and proline cis-trans isomerase activities, as exemplified by the interaction between cyclophilin A (CypA) and the HIV-1 CA protein. Here, we report crystal structures of CypA in complex with HIV-1 CA protein variants that bind preferentially with the substrate proline residue in either the cis or the trans conformation. Cis- and trans-Pro substrates are accommodated within the enzyme active site by rearrangement of their N-terminal residues and with minimal distortions in the path of the main chain. CypA Arg55 guanidinium group probably facilitates catalysis by anchoring the substrate proline oxygen and stabilizing sp3 hybridization of the proline nitrogen in the transition state.
 ==About this Structure==
@@ Line 15: / Line 15: @@
 [[Category: Peptidylprolyl isomerase]]
 [[Category: Protein complex]]
-[[Category: Hill, C.P.]]
+[[Category: Hill, C P.]]
-[[Category: Howard, B.R.]]
+[[Category: Howard, B R.]]
 [[Category: Li, S.]]
-[[Category: Sundquist, W.I.]]
+[[Category: Sundquist, W I.]]
-[[Category: Vajdos, F.F.]]
+[[Category: Vajdos, F F.]]
 [[Category: capsid]]
 [[Category: cyclophilin a]]
@@ Line 26: / Line 26: @@
 [[Category: rotamase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:22:54 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:52:58 2008''