1mel: Difference between revisions

Line 4:

==Overview==

The Camelidae is the only taxonomic family known to possess functional, heavy-chain antibodies, lacking light chains. We report here the 2.5 A, resolution crystal structure of a camel VH in complex with its antigen, lysozyme. Compared to human and mouse VH domains, there are no major, backbone rearrangements in the VH framework. However, the architecture of, the region of VH that interacts with a VL in a conventional FV is, different from any previously seen. Moreover, the CDR1 region, although in, sequence homologous to human CDR1, deviates fundamentally from the, canonical structure. Additionally, one half of the CDR3 contacts the VH, region which in conventional immunoglobulins interacts with a VL whereas, the other half protrudes from the antigen binding site and penetrates, deeply into the active site of lysozyme.

The Camelidae is the only taxonomic family known to possess functional heavy-chain antibodies, lacking light chains. We report here the 2.5 A resolution crystal structure of a camel VH in complex with its antigen, lysozyme. Compared to human and mouse VH domains, there are no major backbone rearrangements in the VH framework. However, the architecture of the region of VH that interacts with a VL in a conventional FV is different from any previously seen. Moreover, the CDR1 region, although in sequence homologous to human CDR1, deviates fundamentally from the canonical structure. Additionally, one half of the CDR3 contacts the VH region which in conventional immunoglobulins interacts with a VL whereas the other half protrudes from the antigen binding site and penetrates deeply into the active site of lysozyme.

==About this Structure==

Line 16:

[[Category: Single protein]]

[[Category: Desmyter, A.]]

[[Category: Gharoudi, M.Arbabi.]]

[[Category: Gharoudi, M Arbabi.]]

[[Category: Hamers, R.]]

[[Category: Muyldermans, S.]]

[[Category: Poortmans, F.]]

[[Category: Thi, M.Dao.]]

[[Category: Thi, M Dao.]]

[[Category: Transue, T.R.]]

[[Category: Transue, T R.]]

[[Category: Wyns, L.]]

[[Category: camel single-domain anti-lysozyme]]

[[Category: complex (antibody/antigen)]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri~~ Feb ~~15 16~~:23:41 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:54:28 2008''

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools

@@ Line 4: / Line 4: @@
 ==Overview==
-The Camelidae is the only taxonomic family known to possess functional, heavy-chain antibodies, lacking light chains. We report here the 2.5 A, resolution crystal structure of a camel VH in complex with its antigen, lysozyme. Compared to human and mouse VH domains, there are no major, backbone rearrangements in the VH framework. However, the architecture of, the region of VH that interacts with a VL in a conventional FV is, different from any previously seen. Moreover, the CDR1 region, although in, sequence homologous to human CDR1, deviates fundamentally from the, canonical structure. Additionally, one half of the CDR3 contacts the VH, region which in conventional immunoglobulins interacts with a VL whereas, the other half protrudes from the antigen binding site and penetrates, deeply into the active site of lysozyme.
+The Camelidae is the only taxonomic family known to possess functional heavy-chain antibodies, lacking light chains. We report here the 2.5 A resolution crystal structure of a camel VH in complex with its antigen, lysozyme. Compared to human and mouse VH domains, there are no major backbone rearrangements in the VH framework. However, the architecture of the region of VH that interacts with a VL in a conventional FV is different from any previously seen. Moreover, the CDR1 region, although in sequence homologous to human CDR1, deviates fundamentally from the canonical structure. Additionally, one half of the CDR3 contacts the VH region which in conventional immunoglobulins interacts with a VL whereas the other half protrudes from the antigen binding site and penetrates deeply into the active site of lysozyme.
 ==About this Structure==
@@ Line 16: / Line 16: @@
 [[Category: Single protein]]
 [[Category: Desmyter, A.]]
-[[Category: Gharoudi, M.Arbabi.]]
+[[Category: Gharoudi, M Arbabi.]]
 [[Category: Hamers, R.]]
 [[Category: Muyldermans, S.]]
 [[Category: Poortmans, F.]]
-[[Category: Thi, M.Dao.]]
+[[Category: Thi, M Dao.]]
-[[Category: Transue, T.R.]]
+[[Category: Transue, T R.]]
 [[Category: Wyns, L.]]
 [[Category: camel single-domain anti-lysozyme]]
 [[Category: complex (antibody/antigen)]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:23:41 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:54:28 2008''