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==Overview==

Oxidative damage to DNA generates 8-oxo-7,8-dihydro-2'-deoxyguanosine, (8-oxodG). During DNA replication and repair synthesis, 8-oxodG can pair, with cytosine or adenine. The ability to accurately replicate through this, lesion depends on the DNA polymerase. We report the first structure of a, polymerase with a promutagenic DNA lesion, 8-oxodG, in the confines of its, active site. The modified guanine residue is in an anti conformation and, forms Watson-Crick hydrogen bonds with an incoming dCTP. To accommodate, the oxygen at C8, the 5'-phosphate backbone of the templating nucleotide, flips 180 degrees. Thus, the flexibility of the template sugar-phosphate, backbone near the polymerase active site is one parameter that influences, the anti-syn equilibrium of 8-oxodG. Our results provide insights into the, mechanisms employed by polymerases to select the complementary dNTP.

Oxidative damage to DNA generates 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG). During DNA replication and repair synthesis, 8-oxodG can pair with cytosine or adenine. The ability to accurately replicate through this lesion depends on the DNA polymerase. We report the first structure of a polymerase with a promutagenic DNA lesion, 8-oxodG, in the confines of its active site. The modified guanine residue is in an anti conformation and forms Watson-Crick hydrogen bonds with an incoming dCTP. To accommodate the oxygen at C8, the 5'-phosphate backbone of the templating nucleotide flips 180 degrees. Thus, the flexibility of the template sugar-phosphate backbone near the polymerase active site is one parameter that influences the anti-syn equilibrium of 8-oxodG. Our results provide insights into the mechanisms employed by polymerases to select the complementary dNTP.

==About this Structure==

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[[Category: Homo sapiens]]

[[Category: Single protein]]

[[Category: Beard, W.A.]]

[[Category: Beard, W A.]]

[[Category: Grollman, A.P.]]

[[Category: Grollman, A P.]]

[[Category: Krahn, J.M.]]

[[Category: Krahn, J M.]]

[[Category: Miller, H.]]

[[Category: Wilson, S.H.]]

[[Category: Wilson, S H.]]

[[Category: DCP]]

[[Category: MG]]

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[[Category: transferase]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Oxidative damage to DNA generates 8-oxo-7,8-dihydro-2'-deoxyguanosine, (8-oxodG). During DNA replication and repair synthesis, 8-oxodG can pair, with cytosine or adenine. The ability to accurately replicate through this, lesion depends on the DNA polymerase. We report the first structure of a, polymerase with a promutagenic DNA lesion, 8-oxodG, in the confines of its, active site. The modified guanine residue is in an anti conformation and, forms Watson-Crick hydrogen bonds with an incoming dCTP. To accommodate, the oxygen at C8, the 5'-phosphate backbone of the templating nucleotide, flips 180 degrees. Thus, the flexibility of the template sugar-phosphate, backbone near the polymerase active site is one parameter that influences, the anti-syn equilibrium of 8-oxodG. Our results provide insights into the, mechanisms employed by polymerases to select the complementary dNTP.
+Oxidative damage to DNA generates 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG). During DNA replication and repair synthesis, 8-oxodG can pair with cytosine or adenine. The ability to accurately replicate through this lesion depends on the DNA polymerase. We report the first structure of a polymerase with a promutagenic DNA lesion, 8-oxodG, in the confines of its active site. The modified guanine residue is in an anti conformation and forms Watson-Crick hydrogen bonds with an incoming dCTP. To accommodate the oxygen at C8, the 5'-phosphate backbone of the templating nucleotide flips 180 degrees. Thus, the flexibility of the template sugar-phosphate backbone near the polymerase active site is one parameter that influences the anti-syn equilibrium of 8-oxodG. Our results provide insights into the mechanisms employed by polymerases to select the complementary dNTP.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
-[[Category: Beard, W.A.]]
+[[Category: Beard, W A.]]
-[[Category: Grollman, A.P.]]
+[[Category: Grollman, A P.]]
-[[Category: Krahn, J.M.]]
+[[Category: Krahn, J M.]]
 [[Category: Miller, H.]]
-[[Category: Wilson, S.H.]]
+[[Category: Wilson, S H.]]
 [[Category: DCP]]
 [[Category: MG]]
@@ Line 25: / Line 25: @@
 [[Category: transferase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:25:13 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:57:53 2008''