1p2s: Difference between revisions

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==Overview==

Room temperature crystal structures of crosslinked H-Ras bound to GMPPNP, were solved in 50% 2,2,2-trifluoroethanol, 60% 1,6-hexanediol, and 50%, isopropanol. The disordered switch II region of Ras is ordered in the, presence of 2,2,2-trifluoroethanol or 1,6-hexanediol. The overall backbone, conformation of switch II in these organic solvents is the same as in the, Ras-GMPPNP complexes with RalGDS, PI(3) kinase, and RasGAP, indicating a, biologically relevant form. Key polar interactions that stabilize the, ordered switch are enhanced in the presence of hydrophobic cosolvents., These results suggest that hydrophobic solvents can be used in general to, order short biologically relevant segments of disordered regions in, protein crystals by favoring H-bonding interactions between atoms that are, highly solvated and mobile in aqueous solution.

Room temperature crystal structures of crosslinked H-Ras bound to GMPPNP were solved in 50% 2,2,2-trifluoroethanol, 60% 1,6-hexanediol, and 50% isopropanol. The disordered switch II region of Ras is ordered in the presence of 2,2,2-trifluoroethanol or 1,6-hexanediol. The overall backbone conformation of switch II in these organic solvents is the same as in the Ras-GMPPNP complexes with RalGDS, PI(3) kinase, and RasGAP, indicating a biologically relevant form. Key polar interactions that stabilize the ordered switch are enhanced in the presence of hydrophobic cosolvents. These results suggest that hydrophobic solvents can be used in general to order short biologically relevant segments of disordered regions in protein crystals by favoring H-bonding interactions between atoms that are highly solvated and mobile in aqueous solution.

==Disease==

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[[Category: Homo sapiens]]

[[Category: Single protein]]

[[Category: Buhrman, G.K.]]

[[Category: Buhrman, G K.]]

[[Category: Mattos, C.]]

[[Category: Serrano, V.de.]]

[[Category: Serrano, V de.]]

[[Category: ETF]]

[[Category: GNP]]

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[[Category: signaling protein]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Room temperature crystal structures of crosslinked H-Ras bound to GMPPNP, were solved in 50% 2,2,2-trifluoroethanol, 60% 1,6-hexanediol, and 50%, isopropanol. The disordered switch II region of Ras is ordered in the, presence of 2,2,2-trifluoroethanol or 1,6-hexanediol. The overall backbone, conformation of switch II in these organic solvents is the same as in the, Ras-GMPPNP complexes with RalGDS, PI(3) kinase, and RasGAP, indicating a, biologically relevant form. Key polar interactions that stabilize the, ordered switch are enhanced in the presence of hydrophobic cosolvents., These results suggest that hydrophobic solvents can be used in general to, order short biologically relevant segments of disordered regions in, protein crystals by favoring H-bonding interactions between atoms that are, highly solvated and mobile in aqueous solution.
+Room temperature crystal structures of crosslinked H-Ras bound to GMPPNP were solved in 50% 2,2,2-trifluoroethanol, 60% 1,6-hexanediol, and 50% isopropanol. The disordered switch II region of Ras is ordered in the presence of 2,2,2-trifluoroethanol or 1,6-hexanediol. The overall backbone conformation of switch II in these organic solvents is the same as in the Ras-GMPPNP complexes with RalGDS, PI(3) kinase, and RasGAP, indicating a biologically relevant form. Key polar interactions that stabilize the ordered switch are enhanced in the presence of hydrophobic cosolvents. These results suggest that hydrophobic solvents can be used in general to order short biologically relevant segments of disordered regions in protein crystals by favoring H-bonding interactions between atoms that are highly solvated and mobile in aqueous solution.
 ==Disease==
@@ Line 16: / Line 16: @@
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
-[[Category: Buhrman, G.K.]]
+[[Category: Buhrman, G K.]]
 [[Category: Mattos, C.]]
-[[Category: Serrano, V.de.]]
+[[Category: Serrano, V de.]]
 [[Category: ETF]]
 [[Category: GNP]]
@@ Line 25: / Line 25: @@
 [[Category: signaling protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:38:27 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:24:28 2008''