Sandbox Reserved 497: Difference between revisions

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==Mechanism of Action==

While the exact mechanism for the methyl ~~transferase~~ reaction catalyzed by DmdA is unknown

While the exact mechanism for the methyl transfer reaction catalyzed by DmdA is still unknown, a proposed mechanism has recently been published based on the known structural characteristics of DmdA

<ref> Schuller, D.J., Reisch, C.R., Moran, M.A., Whitman, W.B., Lanzilotta, W.N. (2012) Structures of dimethylsulfoniopropinate-dependent demethylase from the marine organism pelagabacter ubique. Protein Sci. 21: 289-298. </ref>. The proposed reaction involves an SN2 intermediates with a concerted methyl and a proton transfer mediated by a water molecule present in the active site. This proposed reaction seems likely due to the location of the active site, which is very acessible by water, as well as the <scene name='Sandbox_Reserved_497/Acidicactivesite/1'>acidic side chains</scene>present in the active site. Additionally DmdA contains a sulfonium atom which

[[Image:DmdA_Mechanism.jpg|thumb|600px|right|The proposed mechanism for the methyl transfer reaction catalyzed by DmdA. This image was obtained directly from Schuller et al.]]

The specific mechanism of DmdA is still being investigated. However, a mechanism was recently proposed <ref> Schuller, D.J., Reisch, C.R., Moran, M.A., Whitman, W.B., Lanzilotta, W.N. (2012) Structures of dimethylsulfoniopropinate-dependent demethylase from the marine organism pelagabacter ubique. Protein Sci. 21: 289-298. </ref>

[[Image:DmdA_Mechanism.jpg|thumb|600px|right|The proposed mechanism for the methyl transfer reaction catalyzed by DmdA. ~~Image~~ obtained directly from Schuller et al.]]

==Possible Applications==

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@@ Line 19: / Line 19: @@
 ==Mechanism of Action==
-While the exact mechanism for the methyl transferase reaction catalyzed by DmdA is unknown
+While the exact mechanism for the methyl transfer reaction catalyzed by DmdA is still unknown, a proposed mechanism has recently been published based on the known structural characteristics of DmdA
+<ref> Schuller, D.J., Reisch, C.R., Moran, M.A., Whitman, W.B., Lanzilotta, W.N. (2012) Structures of dimethylsulfoniopropinate-dependent demethylase from the marine organism pelagabacter ubique. Protein Sci. 21: 289-298. </ref>. The proposed reaction involves an SN2 intermediates with a concerted methyl and a proton transfer mediated by a water molecule present in the active site. This proposed reaction seems likely due to the location of the active site, which is very acessible by water, as well as the <scene name='Sandbox_Reserved_497/Acidicactivesite/1'>acidic side chains</scene>present in the active site. Additionally DmdA contains a sulfonium atom which
+[[Image:DmdA_Mechanism.jpg|thumb|600px|right|The proposed mechanism for the methyl transfer reaction catalyzed by DmdA. This image was obtained directly from Schuller et al.]]
-The specific mechanism of DmdA is still being investigated. However, a mechanism was recently proposed <ref> Schuller, D.J., Reisch, C.R., Moran, M.A., Whitman, W.B., Lanzilotta, W.N. (2012) Structures of dimethylsulfoniopropinate-dependent demethylase from the marine organism pelagabacter ubique. Protein Sci. 21: 289-298. </ref>
-[[Image:DmdA_Mechanism.jpg|thumb|600px|right|The proposed mechanism for the methyl transfer reaction catalyzed by DmdA. Image obtained directly from Schuller et al.]]
 ==Possible Applications==