User:Marvin O'Neal/VlsE: Difference between revisions
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Throughout the course of the disease, IR<sub>6</sub> produces a strong antibody response that can be identified from early to late phases. Applications in diagnostic testing have been identified as a result of this strong immune response and IR<sub>6</sub>’s relative invariability across strains.<ref name="I">PMID:10565920</ref> <ref name="F" /> A C<sub>6</sub> [http://en.wikipedia.org/wiki/ELISA ELISA] test has been developed which uses a 26 amino | Throughout the course of the disease, IR<sub>6</sub> produces a strong antibody response that can be identified from early to late phases. Applications in diagnostic testing have been identified as a result of this strong immune response and IR<sub>6</sub>’s relative invariability across strains.<ref name="I">PMID:10565920</ref> <ref name="F" /> A C<sub>6</sub> [http://en.wikipedia.org/wiki/ELISA ELISA] test has been developed which uses a 26 amino acid synthetic peptide, C<sub>6</sub>, containing the IR<sub>6</sub> sequence. Results show 99% specificity and 100% precision with high sensitivity. In fact, OspA vaccination does not influence C6 specificity; therefore, C<sub>6</sub> ELISA tests are valuable diagnostic tools.<ref name="I" /> The CDC currently recommends a [http://www.cdc.gov/lyme/healthcare/clinician_twotier.html two-step test] incorporating first a polyvalent, whole-cell [http://en.wikipedia.org/wiki/Sonicate sonicate] (WCS) [http://en.wikipedia.org/wiki/Immunofluorescence_assay immunofluorescent assay]. If results are positive, this is followed by IgG and IgM WCS [http://en.wikipedia.org/wiki/Western_blot Western blots] to eliminate false positives.<ref name="J">PMID:21865190</ref> Therefore, this one-step ELISA test presents an accurate and economical alternative to the current two-step model.<ref name="I" /> <br> | ||
==Additional Links== | ==Additional Links== | ||
