1ryf: Difference between revisions

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==Overview==

Rac1b was recently identified in malignant colorectal tumors as an, alternative splice variant of Rac1 containing a 19-amino acid insertion, next to the switch II region. The structures of Rac1b in the GDP- and the, GppNHp-bound forms, determined at a resolution of 1.75 A, reveal that the, insertion induces an open switch I conformation and a highly mobile switch, II. As a consequence, Rac1b has an accelerated GEF-independent GDP/GTP, exchange and an impaired GTP hydrolysis, which is restored partially by, GTPase-activating proteins. Interestingly, Rac1b is able to bind the, GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent, manner, suggesting that the insertion does not completely abolish effector, interaction. The presented study provides insights into the structural and, biochemical mechanism of a self-activating GTPase.

Rac1b was recently identified in malignant colorectal tumors as an alternative splice variant of Rac1 containing a 19-amino acid insertion next to the switch II region. The structures of Rac1b in the GDP- and the GppNHp-bound forms, determined at a resolution of 1.75 A, reveal that the insertion induces an open switch I conformation and a highly mobile switch II. As a consequence, Rac1b has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. Interestingly, Rac1b is able to bind the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. The presented study provides insights into the structural and biochemical mechanism of a self-activating GTPase.

==About this Structure==

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[[Category: Single protein]]

[[Category: Small monomeric GTPase]]

[[Category: Ahmadian, M.R.]]

[[Category: Ahmadian, M R.]]

[[Category: Fiegen, D.]]

[[Category: GDP]]

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[[Category: gtp binding]]

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@@ Line 4: / Line 4: @@
 ==Overview==
-Rac1b was recently identified in malignant colorectal tumors as an, alternative splice variant of Rac1 containing a 19-amino acid insertion, next to the switch II region. The structures of Rac1b in the GDP- and the, GppNHp-bound forms, determined at a resolution of 1.75 A, reveal that the, insertion induces an open switch I conformation and a highly mobile switch, II. As a consequence, Rac1b has an accelerated GEF-independent GDP/GTP, exchange and an impaired GTP hydrolysis, which is restored partially by, GTPase-activating proteins. Interestingly, Rac1b is able to bind the, GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent, manner, suggesting that the insertion does not completely abolish effector, interaction. The presented study provides insights into the structural and, biochemical mechanism of a self-activating GTPase.
+Rac1b was recently identified in malignant colorectal tumors as an alternative splice variant of Rac1 containing a 19-amino acid insertion next to the switch II region. The structures of Rac1b in the GDP- and the GppNHp-bound forms, determined at a resolution of 1.75 A, reveal that the insertion induces an open switch I conformation and a highly mobile switch II. As a consequence, Rac1b has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. Interestingly, Rac1b is able to bind the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. The presented study provides insights into the structural and biochemical mechanism of a self-activating GTPase.
 ==About this Structure==
@@ Line 14: / Line 14: @@
 [[Category: Single protein]]
 [[Category: Small monomeric GTPase]]
-[[Category: Ahmadian, M.R.]]
+[[Category: Ahmadian, M R.]]
 [[Category: Fiegen, D.]]
 [[Category: GDP]]
@@ Line 20: / Line 20: @@
 [[Category: gtp binding]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:50:54 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:55:53 2008''