1rzb: Difference between revisions

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==Overview==

Metal-substituted crystals of human carbonic anhydrase II belonging to, space group P2(1) with cell dimensions a = 42.7, b = 41.7, c = 73.0 A and, beta = 104.6 degrees were analyzed crystallographically. The resolution, limit ranged from 1.82 to 1.92 A with high completeness (86.2-90.7%)., Cobalt(II)-substituted carbonic anhydrase has a tetrahedral coordination, around the metal both at pH 6 and pH 7.8, similar to the native zinc, enzyme. In contrast, the catalytically inactive copper(II), nickel(II) and, manganese(II) derivatives showed increased coordination number around the, metal ion. Whereas the copper is best described as penta-coordinated, the, nickel and manganese are best described as hexa-coordinated. The results, are briefly compared with spectroscopic observations and our current view, on carbonic anhydrase catalysis.

Metal-substituted crystals of human carbonic anhydrase II belonging to space group P2(1) with cell dimensions a = 42.7, b = 41.7, c = 73.0 A and beta = 104.6 degrees were analyzed crystallographically. The resolution limit ranged from 1.82 to 1.92 A with high completeness (86.2-90.7%). Cobalt(II)-substituted carbonic anhydrase has a tetrahedral coordination around the metal both at pH 6 and pH 7.8, similar to the native zinc enzyme. In contrast, the catalytically inactive copper(II), nickel(II) and manganese(II) derivatives showed increased coordination number around the metal ion. Whereas the copper is best described as penta-coordinated, the nickel and manganese are best described as hexa-coordinated. The results are briefly compared with spectroscopic observations and our current view on carbonic anhydrase catalysis.

==Disease==

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[[Category: lyase(oxo-acid)]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:56:10 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-Metal-substituted crystals of human carbonic anhydrase II belonging to, space group P2(1) with cell dimensions a = 42.7, b = 41.7, c = 73.0 A and, beta = 104.6 degrees were analyzed crystallographically. The resolution, limit ranged from 1.82 to 1.92 A with high completeness (86.2-90.7%)., Cobalt(II)-substituted carbonic anhydrase has a tetrahedral coordination, around the metal both at pH 6 and pH 7.8, similar to the native zinc, enzyme. In contrast, the catalytically inactive copper(II), nickel(II) and, manganese(II) derivatives showed increased coordination number around the, metal ion. Whereas the copper is best described as penta-coordinated, the, nickel and manganese are best described as hexa-coordinated. The results, are briefly compared with spectroscopic observations and our current view, on carbonic anhydrase catalysis.
+Metal-substituted crystals of human carbonic anhydrase II belonging to space group P2(1) with cell dimensions a = 42.7, b = 41.7, c = 73.0 A and beta = 104.6 degrees were analyzed crystallographically. The resolution limit ranged from 1.82 to 1.92 A with high completeness (86.2-90.7%). Cobalt(II)-substituted carbonic anhydrase has a tetrahedral coordination around the metal both at pH 6 and pH 7.8, similar to the native zinc enzyme. In contrast, the catalytically inactive copper(II), nickel(II) and manganese(II) derivatives showed increased coordination number around the metal ion. Whereas the copper is best described as penta-coordinated, the nickel and manganese are best described as hexa-coordinated. The results are briefly compared with spectroscopic observations and our current view on carbonic anhydrase catalysis.
 ==Disease==
@@ Line 24: / Line 24: @@
 [[Category: lyase(oxo-acid)]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:51:02 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:56:10 2008''