Sandbox Reserved 508: Difference between revisions
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(navy). Additional positively charged residues in the interior of the barrel may pass the fatty acid anion | (navy). Additional positively charged residues in the interior of the barrel may pass the fatty acid anion | ||
towards the inter membrane space.]] | towards the inter membrane space.]] | ||
[[Image:BCSMART_11-12_Figure_7.JPG|right|375px|thumb|'''FIGURE 7: The Effect of UCP2 on ROS Production''' | |||
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The data above shows the effect of UCP2 on ROS production. The ROS is made visible by DCF fluorescence(dichloro-fluorescein, which fluoresces in the presence of H2O2). ROS species are introduced into neonatal rat cardiac ventricular myocytes by injecting H2O2. | |||
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A. Confocal microscope images of cardiomyocytes’ ROS production | |||
Top row: ROS in myoctes which have been exposed to adenovirus without the UCP2 protein. Increased DCF fluorescence can be seen over time, representing the presence of a significant level of ROS. Bottom row: ROS in a myocytes exposed to an adenovirus producing the UCP2 protein.DCF fluorescence is barely visible, representing little to no ROS presence. | |||
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B. Quantitative representation of the changes in ROS levels corresponding to the fluorescence images in A using time-lapse confocal microscopy. | |||
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The presence of UCP2 greatly reduces ROS levels, limiting damage to cardiac ventricular myocytes.]] | |||
