Protein PA4608: Difference between revisions
New page: left|200px <!-- The line below this paragraph, containing "STRUCTURE_1ywu", creates the "Structure Box" on the page. You may change the PDB parameter (which sets the PD... |
No edit summary |
||
| Line 16: | Line 16: | ||
(as it appears on PubMed at http://www.pubmed.gov), where 17096419 is the PubMed ID number. | (as it appears on PubMed at http://www.pubmed.gov), where 17096419 is the PubMed ID number. | ||
--> | --> | ||
<scene name='1ywu/Chain/2'>Chain</scene> | <scene name='1ywu/Chain/2'>Chain</scene> | ||
<scene name='1ywu/Bindingsite/3'>C-di-GMP binding site</scene> | <scene name='1ywu/Bindingsite/3'>C-di-GMP binding site</scene> | ||
==PA4608 PilZ domain== | |||
<scene name='1ywu/Pilz_active/1'>PilZ active site</scene> (R9, R13, D35, S37, G40) | |||
{{ABSTRACT_PUBMED_17096419}} | |||
==About this Structure== | ==About this Structure== | ||
| Line 45: | Line 48: | ||
[[Category: C-di-GMP]] | [[Category: C-di-GMP]] | ||
[[Category: Structural genomic]] | [[Category: Structural genomic]] | ||
<br /> | |||
Created with the participation of [[User:Tilman Schirmer|Tilman Schirmer]]. | |||
< | |||
Revision as of 10:19, 6 June 2012
Solution NMR structure of Pseudomonas Aeruginosa protein PA4608. Northeast Structural Genomics target PaT7
PA4608 PilZ domain
PilZ active site (R9, R13, D35, S37, G40)
PA4608 is a 125 residue protein from Pseudomonas aeruginosa with a recent identification as a PilZ domain and putative bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) adaptor protein that plays a role in bacterial second-messenger regulated processes. The nuclear magnetic resonance (NMR) structure of PA4608 has been determined and c-di-GMP binding has been confirmed by NMR titration studies. The monomeric core structure of PA4608 contains a six-stranded anti-parallel beta barrel flanked by three helices. Conserved surface residues among PA4608 homologs suggest the c-di-GMP binding site is at one end of the barrel and includes residues in the helices as well as in the unstructured N-terminus. Chemical shift changes in PA4608 resonances upon titration with c-di-GMP confirm binding. This evidence supports the hypothesis that proteins containing PilZ domains are the long-sought c-di-GMP adaptor proteins.
NMR structure and binding studies confirm that PA4608 from Pseudomonas aeruginosa is a PilZ domain and a c-di-GMP binding protein., Ramelot TA, Yee A, Cort JR, Semesi A, Arrowsmith CH, Kennedy MA, Proteins. 2007 Feb 1;66(2):266-71. PMID:17096419
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
1YWU is a 1 chain structure of sequence from Pseudomonas aeruginosa pao1. Full experimental information is available from OCA.
Reference
NMR structure and binding studies confirm that PA4608 from Pseudomonas aeruginosa is a PilZ domain and a c-di-GMP binding protein., Ramelot TA, Yee A, Cort JR, Semesi A, Arrowsmith CH, Kennedy MA, Proteins. 2007 Feb 1;66(2):266-71. PMID:17096419
Created with the participation of Tilman Schirmer.
