2ren: Difference between revisions

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==Overview==

The x-ray crystal structure of recombinant human renin has been, determined. Molecular dynamics techniques that included crystallographic, data as a restraint were used to improve an initial model based on porcine, pepsinogen. The present agreement factor for data from 8.0 to 2.5, angstroms (A) is 0.236. Some of the surface loops are poorly determined, and these disordered regions border a 30 A wide solvent channel., Comparison of renin with other aspartyl proteinases shows that, although, the structural cores and active sites are highly conserved, surface, residues, some of which are critical for specificity, vary greatly (up to, 10A). Knowledge of the actual structure, as opposed to the use of models, based on related enzymes, should facilitate the design of renin, inhibitors.

The x-ray crystal structure of recombinant human renin has been determined. Molecular dynamics techniques that included crystallographic data as a restraint were used to improve an initial model based on porcine pepsinogen. The present agreement factor for data from 8.0 to 2.5 angstroms (A) is 0.236. Some of the surface loops are poorly determined, and these disordered regions border a 30 A wide solvent channel. Comparison of renin with other aspartyl proteinases shows that, although the structural cores and active sites are highly conserved, surface residues, some of which are critical for specificity, vary greatly (up to 10A). Knowledge of the actual structure, as opposed to the use of models based on related enzymes, should facilitate the design of renin inhibitors.

==Disease==

Line 17:

[[Category: Renin]]

[[Category: Single protein]]

[[Category: James, M.N.G.]]

[[Category: James, M N.G.]]

[[Category: Sielecki, A.R.]]

[[Category: Sielecki, A R.]]

[[Category: NAG]]

[[Category: hydrolase(acid proteinase)]]

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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:46:42 2008''

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@@ Line 4: / Line 4: @@
 ==Overview==
-The x-ray crystal structure of recombinant human renin has been, determined. Molecular dynamics techniques that included crystallographic, data as a restraint were used to improve an initial model based on porcine, pepsinogen. The present agreement factor for data from 8.0 to 2.5, angstroms (A) is 0.236. Some of the surface loops are poorly determined, and these disordered regions border a 30 A wide solvent channel., Comparison of renin with other aspartyl proteinases shows that, although, the structural cores and active sites are highly conserved, surface, residues, some of which are critical for specificity, vary greatly (up to, 10A). Knowledge of the actual structure, as opposed to the use of models, based on related enzymes, should facilitate the design of renin, inhibitors.
+The x-ray crystal structure of recombinant human renin has been determined. Molecular dynamics techniques that included crystallographic data as a restraint were used to improve an initial model based on porcine pepsinogen. The present agreement factor for data from 8.0 to 2.5 angstroms (A) is 0.236. Some of the surface loops are poorly determined, and these disordered regions border a 30 A wide solvent channel. Comparison of renin with other aspartyl proteinases shows that, although the structural cores and active sites are highly conserved, surface residues, some of which are critical for specificity, vary greatly (up to 10A). Knowledge of the actual structure, as opposed to the use of models based on related enzymes, should facilitate the design of renin inhibitors.
 ==Disease==
@@ Line 17: / Line 17: @@
 [[Category: Renin]]
 [[Category: Single protein]]
-[[Category: James, M.N.G.]]
+[[Category: James, M N.G.]]
-[[Category: Sielecki, A.R.]]
+[[Category: Sielecki, A R.]]
 [[Category: NAG]]
 [[Category: hydrolase(acid proteinase)]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:42:04 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:46:42 2008''