P53-DNA Recognition: Difference between revisions
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==Structural Description of p53-DNA Complex== | ==Structural Description of p53-DNA Complex== | ||
=Domain Architecture and Tetramerization= | ===Domain Architecture and Tetramerization=== | ||
<Structure load='3kz8bio.pdb.zip' size='500' frame='true' align='right' caption='Figure 5: Crystal structure of p53 DBD tetramer-DNA complex, PDB ID 3KZ8.' scene='Sandbox_Reserved_170/Complex/6' /> | <Structure load='3kz8bio.pdb.zip' size='500' frame='true' align='right' caption='Figure 5: Crystal structure of p53 DBD tetramer-DNA complex, PDB ID 3KZ8.' scene='Sandbox_Reserved_170/Complex/6' /> | ||
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The p53 DBD assumes the conformation of an <scene name='Sandbox_Reserved_170/Beta/1'>immunoglobulin-like fold consisting of a beta sandwich</scene>, which binds the response element in the major groove. A functionally important <scene name='Sandbox_Reserved_170/Zn/1'>Zn2+ ion coordinates the Cys176, His179, Cys238, Cys242 residues</scene> and, thus, stabilizes the fold of the DBD. | The p53 DBD assumes the conformation of an <scene name='Sandbox_Reserved_170/Beta/1'>immunoglobulin-like fold consisting of a beta sandwich</scene>, which binds the response element in the major groove. A functionally important <scene name='Sandbox_Reserved_170/Zn/1'>Zn2+ ion coordinates the Cys176, His179, Cys238, Cys242 residues</scene> and, thus, stabilizes the fold of the DBD. | ||
=Protein-Protein Interactions= | ===Protein-Protein Interactions=== | ||
The p53 tetramer forms a relatively small <scene name='Sandbox_Reserved_170/Intra-dimer/4'>intra-dimer with two salt bridges between Glu180 and Arg181 residues</scene> and, in comparison, a large <scene name='Sandbox_Reserved_170/Inter-dimer/4'>inter-dimer interface with an extensive network of interactions</scene>. The actual molecular interactions and strength in binding can vary as a function of the sequence and spacer length of the response element. | The p53 tetramer forms a relatively small <scene name='Sandbox_Reserved_170/Intra-dimer/4'>intra-dimer with two salt bridges between Glu180 and Arg181 residues</scene> and, in comparison, a large <scene name='Sandbox_Reserved_170/Inter-dimer/4'>inter-dimer interface with an extensive network of interactions</scene>. The actual molecular interactions and strength in binding can vary as a function of the sequence and spacer length of the response element. | ||
===Major Groove Base Readout=== | |||
<scene name='Sandbox_Reserved_170/Arg280_contact/5'>Arg280 contact</scene> | <scene name='Sandbox_Reserved_170/Arg280_contact/5'>Arg280 contact</scene> | ||
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<scene name='Sandbox_Reserved_170/Arg273/2'>Arg273</scene> | <scene name='Sandbox_Reserved_170/Arg273/2'>Arg273</scene> | ||
===Minor Groove Shape Readout=== | |||
<scene name='Sandbox_Reserved_170/Arg248/2'>Arg248</scene> | <scene name='Sandbox_Reserved_170/Arg248/2'>Arg248</scene> | ||
