4f1n: Difference between revisions
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[[ | ==Crystal structure of Kluyveromyces polysporus Argonaute with a guide RNA== | ||
<StructureSection load='4f1n' size='340' side='right' caption='[[4f1n]], [[Resolution|resolution]] 3.19Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4f1n]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Vanderwaltozyma_polyspora Vanderwaltozyma polyspora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F1N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F1N FirstGlance]. <br> | |||
</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Kpol_520p25 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36033 Vanderwaltozyma polyspora])</td></tr> | |||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f1n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f1n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f1n RCSB], [http://www.ebi.ac.uk/pdbsum/4f1n PDBsum]</span></td></tr> | |||
<table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The RNA-induced silencing complex, comprising Argonaute and guide RNA, mediates RNA interference. Here we report the 3.2 A crystal structure of Kluyveromyces polysporus Argonaute (KpAGO) fortuitously complexed with guide RNA originating from small-RNA duplexes autonomously loaded by recombinant KpAGO. Despite their diverse sequences, guide-RNA nucleotides 1-8 are positioned similarly, with sequence-independent contacts to bases, phosphates and 2'-hydroxyl groups pre-organizing the backbone of nucleotides 2-8 in a near-A-form conformation. Compared with prokaryotic Argonautes, KpAGO has numerous surface-exposed insertion segments, with a cluster of conserved insertions repositioning the N domain to enable full propagation of guide-target pairing. Compared with Argonautes in inactive conformations, KpAGO has a hydrogen-bond network that stabilizes an expanded and repositioned loop, which inserts an invariant glutamate into the catalytic pocket. Mutation analyses and analogies to ribonuclease H indicate that insertion of this glutamate finger completes a universally conserved catalytic tetrad, thereby activating Argonaute for RNA cleavage. | |||
Structure of yeast Argonaute with guide RNA.,Nakanishi K, Weinberg DE, Bartel DP, Patel DJ Nature. 2012 Jun 20;486(7403):368-74. doi: 10.1038/nature11211. PMID:22722195<ref>PMID:22722195</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Vanderwaltozyma polyspora]] | [[Category: Vanderwaltozyma polyspora]] | ||
[[Category: Bartel, D P.]] | [[Category: Bartel, D P.]] | ||
Revision as of 08:08, 5 June 2014
Crystal structure of Kluyveromyces polysporus Argonaute with a guide RNA
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