111d: Difference between revisions
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'''CRYSTAL STRUCTURE AND STABILITY OF A DNA DUPLEX CONTAINING A(ANTI).G(SYN) BASE-PAIRS''' | {{Structure | ||
|PDB= 111d |SIZE=350|CAPTION= <scene name='initialview01'>111d</scene>, resolution 2.250Å | |||
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'''CRYSTAL STRUCTURE AND STABILITY OF A DNA DUPLEX CONTAINING A(ANTI).G(SYN) BASE-PAIRS''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
111D is a [ | 111D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=111D OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs., Brown T, Leonard GA, Booth ED, Chambers J, J Mol Biol. 1989 May 20;207(2):455-7. PMID:[http:// | Crystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs., Brown T, Leonard GA, Booth ED, Chambers J, J Mol Biol. 1989 May 20;207(2):455-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2754734 2754734] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Booth, E D.]] | [[Category: Booth, E D.]] | ||
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[[Category: mismatched]] | [[Category: mismatched]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:48:34 2008'' | ||
Revision as of 07:48, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
CRYSTAL STRUCTURE AND STABILITY OF A DNA DUPLEX CONTAINING A(ANTI).G(SYN) BASE-PAIRS
Overview
The synthetic dodecanucleotide d(CGCAAATTGGCG) has been analysed by single-crystal X-ray diffraction techniques and the structure refined to R = 0.16 and 2.25 A resolution, with the location of 94 solvent molecules. The sequence crystallizes as a full turn of a B-DNA helix with ten Watson-Crick base-pairs and two adenine-guanine mispairs. The analysis clearly shows that the mismatches are of the form A(anti).G(syn). Thermal denaturation studies indicate that the stability of the duplex is strongly pH dependent, with a maximum at pH 5.0, suggesting that the base-pair is stabilized by protonation. Three different arrangements have been observed for base-pairs between guanine and adenine and it is likely that A.G mismatch conformation is strongly influenced by dipole-dipole interactions with adjacent base-pairs.
About this Structure
111D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure and stability of a DNA duplex containing A(anti).G(syn) base-pairs., Brown T, Leonard GA, Booth ED, Chambers J, J Mol Biol. 1989 May 20;207(2):455-7. PMID:2754734
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