Prp8: Difference between revisions

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==Prp8 Background==

Prp8's name originates from the discovery that it is intricately involved with ''[[p]]''re-m ''[[R]]''NA ''[[p]]''rocessing within Eukaryotic organisms, and extensive research on the protein has revealed it is indispensable for the catalytic activity of the spliceosome earning it the nickname of 'Master Regulator of the Spliceosome' <ref name='Prp8: At the heart of the spliceosome'>DOI:10.1261/rna.2220705</ref>. UV crosslinking experiments have shown that Prp8 has contact with the 5' splice site, 3' splice site, [http://en.wikipedia.org/wiki/Polypyrimidine_tract polypyrimidine tract], and branch point of pre-mRNA transcripts, as well as associating with all five of the snRNAs and their associated proteins <ref name='Functional interactions of prp8 with both splice sites at the spliceosome catalytic center'>PMID:10444596</ref>. Thus, Prp8 is the only protein to date involved with [http://en.wikipedia.org/wiki/RNA_splicing pre-mRNA splicing] that actually contacts all catalytic elements of the spliceosome, indicating that it indeed has a very crucial role in pre-mRNA splicing catalysis, and resides in the catalytic heart of the splicing comlex. Two other unique properties of Prp8 are its size and evolutionary conservation. ~~The protein~~ is approximately 230 - 280 kDa and is approximately ~~2335~~ - ~~2413~~ amino acid residues in length (depending on the ~~species~~) <ref name='Prp8: At the heart of the spliceosome'>DOI:10.1261/rna.2220705</ref>.

Prp8's name originates from the discovery that it is intricately involved with ''[[p]]''re-m ''[[R]]''NA ''[[p]]''rocessing within Eukaryotic organisms, and extensive research on the protein has revealed it is indispensable for the catalytic activity of the spliceosome earning it the nickname of 'Master Regulator of the Spliceosome' <ref name='Prp8: At the heart of the spliceosome'>DOI:10.1261/rna.2220705</ref>. UV crosslinking experiments have shown that Prp8 has contact with the 5' splice site, 3' splice site, [http://en.wikipedia.org/wiki/Polypyrimidine_tract polypyrimidine tract], and branch point of pre-mRNA transcripts, as well as associating with all five of the snRNAs and their associated proteins <ref name='Functional interactions of prp8 with both splice sites at the spliceosome catalytic center'>PMID:10444596</ref>. Thus, Prp8 is the only protein to date involved with [http://en.wikipedia.org/wiki/RNA_splicing pre-mRNA splicing] that actually contacts all catalytic elements of the spliceosome, indicating that it indeed has a very crucial role in pre-mRNA splicing catalysis, and resides in the catalytic heart of the splicing comlex. Two other unique properties of Prp8 are its size and evolutionary conservation. Prp8 is the largest of all the spliceosomal proteins at approximately 230 - 280 kDa and is approximately 2317 - 2416 amino acid residues in length (depending on the orthologue) <ref name='Prp8: At the heart of the spliceosome'>DOI:10.1261/rna.2220705</ref>. In all organisms that exhibit Prp8, it is always found as a U5 snRNP component and is highly conserved across species, with >60% identity between fungi, mammals, and plants <ref name='Prp8, the pivotal protein of the spliceosomal catalytic center, evolved from a retroelement-encoded reverse transcriptase'>PMID:21441348 </ref>.

==Pre-mRNA Splicing==

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@@ Line 8: / Line 8: @@
 ==Prp8 Background==
-Prp8's name originates from the discovery that it is intricately involved with ''[[p]]''re-m ''[[R]]''NA ''[[p]]''rocessing  within Eukaryotic organisms, and extensive research on the protein has revealed it is indispensable for the catalytic activity of the spliceosome earning it the nickname of 'Master Regulator of the Spliceosome' <ref name='Prp8: At the heart of the spliceosome'>DOI:10.1261/rna.2220705</ref>. UV crosslinking experiments have shown that Prp8 has contact with the 5' splice site, 3' splice site, [http://en.wikipedia.org/wiki/Polypyrimidine_tract polypyrimidine tract], and branch point of pre-mRNA transcripts, as well as associating with all five of the snRNAs and their associated proteins <ref name='Functional interactions of prp8 with both splice sites at the spliceosome catalytic center'>PMID:10444596</ref>. Thus, Prp8 is the only protein to date involved with [http://en.wikipedia.org/wiki/RNA_splicing pre-mRNA splicing] that actually contacts all catalytic elements of the spliceosome, indicating that it indeed has a very crucial role in pre-mRNA splicing catalysis, and resides in the catalytic heart of the splicing comlex. Two other unique properties of Prp8 are its size and evolutionary conservation. The protein is approximately 230 - 280 kDa and is approximately 2335 - 2413 amino acid residues in length (depending on the species) <ref name='Prp8: At the heart of the spliceosome'>DOI:10.1261/rna.2220705</ref>.
+Prp8's name originates from the discovery that it is intricately involved with ''[[p]]''re-m ''[[R]]''NA ''[[p]]''rocessing  within Eukaryotic organisms, and extensive research on the protein has revealed it is indispensable for the catalytic activity of the spliceosome earning it the nickname of 'Master Regulator of the Spliceosome' <ref name='Prp8: At the heart of the spliceosome'>DOI:10.1261/rna.2220705</ref>. UV crosslinking experiments have shown that Prp8 has contact with the 5' splice site, 3' splice site, [http://en.wikipedia.org/wiki/Polypyrimidine_tract polypyrimidine tract], and branch point of pre-mRNA transcripts, as well as associating with all five of the snRNAs and their associated proteins <ref name='Functional interactions of prp8 with both splice sites at the spliceosome catalytic center'>PMID:10444596</ref>. Thus, Prp8 is the only protein to date involved with [http://en.wikipedia.org/wiki/RNA_splicing pre-mRNA splicing] that actually contacts all catalytic elements of the spliceosome, indicating that it indeed has a very crucial role in pre-mRNA splicing catalysis, and resides in the catalytic heart of the splicing comlex. Two other unique properties of Prp8 are its size and evolutionary conservation. Prp8 is the largest of all the spliceosomal proteins at approximately 230 - 280 kDa and is approximately 2317 - 2416 amino acid residues in length (depending on the orthologue) <ref name='Prp8: At the heart of the spliceosome'>DOI:10.1261/rna.2220705</ref>. In all organisms that exhibit Prp8, it is always found as a U5 snRNP component and is highly conserved across species, with >60% identity between fungi, mammals, and plants <ref name='Prp8, the pivotal protein of the spliceosomal catalytic center, evolved from a retroelement-encoded reverse transcriptase'>PMID:21441348 </ref>.
 ==Pre-mRNA Splicing==