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| ==='''How does Kapβ2 release its cargo?'''=== | | ==='''How does Kapβ2 release its cargo?'''=== |
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| '''Passage of Kapβ2 Through the NPC4'''
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| Once the cargo binds to Kapβ2, the complex travels through the NPC by hydrophobic interactions with the FG-Nups.
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| These interactions occur at two binding sites: one located between HEAT repeat 5 and 6, and the other between HEAT 6 and 7.
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| '''Dissociation of NLS5'''
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| Ran is a GTP binding protein that is found in greater concentrations in the nucleus than in the cytoplasm.
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| RanGTP has an overall positive charge allowing it to bind to the core of the N-terminal arch of the highly negative Kapβ2.
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| [[Image:Polar Interaction Chart.jpg|left|thumb|alt= Alt text| '''Figure#.''' Chart showing polar interactions between residues of Kapβ2 and Ran3. Negatively charged residues are highlighted in red and positively charged residues are highlighted in blue.
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| |275px]]
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| RanGTP binds to Kapβ2 at its N-terminal arch, beginning at HEAT repeat 1 through 7. This results in conformational changes not only within the N-terminal arch but also within a long (61 residue) loop of HEAT repeat 8, which is thought to be the cause of the dissociation of the cargo.
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