Sandbox 122: Difference between revisions
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The NLSs located on Kapβ2 cargos are named the PY- NLS and they bind to the C-terminal arch of Kapβ2. | The NLSs located on Kapβ2 cargos are named the PY- NLS and they bind to the C-terminal arch of Kapβ2. | ||
Recognition of the PY-NLS by Kapβ2 follows certain guidelines: | Recognition of the PY-NLS by Kapβ2 follows certain guidelines: | ||
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(ii) PY-NLS has an overall positive charge allowing for electrostatic compatibility with Kapβ2. | (ii) PY-NLS has an overall positive charge allowing for electrostatic compatibility with Kapβ2. | ||
(iii) General sequence for the PY-NLS is either a hydrophobic or basic motif at the N- terminus and a R-X2-5-P-Y motif at the C- terminus. | (iii) General sequence for the PY-NLS is either a hydrophobic or basic motif at the N-terminus and a R-X2-5-P-Y motif at the C-terminus. | ||
The PY-NLS of 2H4M contains a hydrophobic rather than a basic N- terminal motif. Hydrophobic interactions at the N- terminal motif of the PY- NLS include: Phe273, Gly274, Pro275 and Met276 of the NLS with Trp730 and Ile773 of Kapβ2. Interactions at the C- terminal R-X2-5-P-Y motif of the NLS include: Arg284 of the NLS with Glu509 and Asp543 of Kapβ2; Pro288 and Tyr289 of the NLS with Ala380, Ala 381, Ala 384, Leu419, Ile457, Trp460 and Arg464 of Kapβ2. | The PY-NLS of 2H4M contains a hydrophobic rather than a basic N- terminal motif. Hydrophobic interactions at the N- terminal motif of the PY- NLS include: Phe273, Gly274, Pro275 and Met276 of the NLS with Trp730 and Ile773 of Kapβ2. Interactions at the C- terminal R-X2-5-P-Y motif of the NLS include: Arg284 of the NLS with Glu509 and Asp543 of Kapβ2; Pro288 and Tyr289 of the NLS with Ala380, Ala 381, Ala 384, Leu419, Ile457, Trp460 and Arg464 of Kapβ2. | ||
