3miw: Difference between revisions
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[[ | ==Crystal Structure of Rotavirus NSP4== | ||
<StructureSection load='3miw' size='340' side='right' caption='[[3miw]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3miw]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Rotavirus_g4 Rotavirus g4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MIW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MIW FirstGlance]. <br> | |||
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene><br> | |||
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2o1j|2o1j]], [[2o1k|2o1k]]</td></tr> | |||
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">G10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=35336 Rotavirus G4])</td></tr> | |||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3miw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3miw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3miw RCSB], [http://www.ebi.ac.uk/pdbsum/3miw PDBsum]</span></td></tr> | |||
<table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The region spanning residues 95-146 of the rotavirus nonstructural protein NSP4 from the asymptomatic human strain ST3 has been purified and crystallized and diffraction data have been collected to a resolution of 2.6 A. Several attempts to solve the structure by the molecular-replacement method using the available tetrameric structures of this domain were unsuccessful despite a sequence identity of 73% to the already known structures. A more systematic approach with a dimer as the search model led to an unexpected pentameric structure using the program Phaser. The various steps involved in arriving at this molecular-replacement solution, which unravelled a case of subtle variation between different oligomeric states unknown at the time of solving the structure, are presented in this paper. | |||
A new pentameric structure of rotavirus NSP4 revealed by molecular replacement.,Chacko AR, Jeyakanthan J, Ueno G, Sekar K, Rao CD, Dodson EJ, Suguna K, Read RJ Acta Crystallogr D Biol Crystallogr. 2012 Jan;68(Pt 1):57-61. Epub 2011 Dec 9. PMID:22194333<ref>PMID:22194333</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
== | |||
< | |||
[[Category: Rotavirus g4]] | [[Category: Rotavirus g4]] | ||
[[Category: Chacko, A R.]] | [[Category: Chacko, A R.]] | ||
Revision as of 10:40, 28 May 2014
Crystal Structure of Rotavirus NSP4
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