1ana: Difference between revisions
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'''HELIX GEOMETRY AND HYDRATION IN AN A-DNA TETRAMER. IC-C-G-G''' | {{Structure | ||
|PDB= 1ana |SIZE=350|CAPTION= <scene name='initialview01'>1ana</scene>, resolution 2.000Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
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'''HELIX GEOMETRY AND HYDRATION IN AN A-DNA TETRAMER. IC-C-G-G''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1ANA is a [ | 1ANA is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. The following page contains interesting information on the relation of 1ANA with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb23_1.html DNA]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ANA OCA]. | ||
==Reference== | ==Reference== | ||
Helix geometry and hydration in an A-DNA tetramer: IC-C-G-G., Conner BN, Yoon C, Dickerson JL, Dickerson RE, J Mol Biol. 1984 Apr 25;174(4):663-95. PMID:[http:// | Helix geometry and hydration in an A-DNA tetramer: IC-C-G-G., Conner BN, Yoon C, Dickerson JL, Dickerson RE, J Mol Biol. 1984 Apr 25;174(4):663-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/6726797 6726797] | ||
[[Category: DNA]] | [[Category: DNA]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: modified]] | [[Category: modified]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:59:51 2008'' | ||
Revision as of 07:59, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
HELIX GEOMETRY AND HYDRATION IN AN A-DNA TETRAMER. IC-C-G-G
Overview
The DNA oligomer of sequence IC-C-G-G has been synthesized, and its X-ray crystal structure solved at a resolution of 2.0 A, using anomalous scattering from iodines in phase analysis: 48 cycles of Jack-Levitt restrained least-squares refinement resulted in a residual error of 19.9% over all data, or 16.5% for two-sigma data. Two double-helical tetramers stack in the crystal to form a continuous octamer, except for the two missing phosphate connections across the center. The octamer has a mean helix rotation of 33.7 degrees (10.7 base-pairs per turn), rise of 2.87 A, mean inclination angle of base-pairs of 14 degrees, and mean base-pair propeller twist of +16.3 degrees. Local variations in both helix rotation and base plane roll angles, including those across the center of the octamer, are as predicted from base sequence by sum functions sigma 1 and sigma 2. The three known DNA octamers: IC-C-G-G/IC-C-G-G, G-G-T-A-T-A-C-C and G-G-C-C-G-G-C-C, make up a graded series in this order, with monotonically changing structural parameters. An exhaustive comparison of torsion angle correlations among the known A helices confirms some structural expectations and reveals some new features. 86 water molecules have been located per double-helical IC-C-G-G tetramer (the asymmetric unit), of which 451/2 per tetramer lie within a first hydrogen-bonded shell of hydration. No ordered water structure is observed comparable to the minor groove spine of hydration in B-DNA.
About this Structure
1ANA is a Protein complex structure of sequences from [1]. The following page contains interesting information on the relation of 1ANA with [DNA]. Full crystallographic information is available from OCA.
Reference
Helix geometry and hydration in an A-DNA tetramer: IC-C-G-G., Conner BN, Yoon C, Dickerson JL, Dickerson RE, J Mol Biol. 1984 Apr 25;174(4):663-95. PMID:6726797
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