1bf3: Difference between revisions

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[[Image:1bf3.gif|left|200px]]<br /><applet load="1bf3" size="350" color="white" frame="true" align="right" spinBox="true"
[[Image:1bf3.gif|left|200px]]
caption="1bf3, resolution 2.2&Aring;" />
 
'''P-HYDROXYBENZOATE HYDROXYLASE (PHBH) MUTANT WITH CYS 116 REPLACED BY SER (C116S) AND ARG 42 REPLACED BY LYS (R42K), IN COMPLEX WITH FAD AND 4-HYDROXYBENZOIC ACID'''<br />
{{Structure
|PDB= 1bf3 |SIZE=350|CAPTION= <scene name='initialview01'>1bf3</scene>, resolution 2.2&Aring;
|SITE=
|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> and <scene name='pdbligand=PHB:P-HYDROXYBENZOIC ACID'>PHB</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/4-hydroxybenzoate_3-monooxygenase 4-hydroxybenzoate 3-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.2 1.14.13.2]
|GENE= POBA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 Pseudomonas fluorescens])
}}
 
'''P-HYDROXYBENZOATE HYDROXYLASE (PHBH) MUTANT WITH CYS 116 REPLACED BY SER (C116S) AND ARG 42 REPLACED BY LYS (R42K), IN COMPLEX WITH FAD AND 4-HYDROXYBENZOIC ACID'''
 


==Overview==
==Overview==
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==About this Structure==
==About this Structure==
1BF3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens] with <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=PHB:'>PHB</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/4-hydroxybenzoate_3-monooxygenase 4-hydroxybenzoate 3-monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.2 1.14.13.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BF3 OCA].  
1BF3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BF3 OCA].  


==Reference==
==Reference==
Lys42 and Ser42 variants of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens reveal that Arg42 is essential for NADPH binding., Eppink MH, Schreuder HA, van Berkel WJ, Eur J Biochem. 1998 Apr 1;253(1):194-201. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9578477 9578477]
Lys42 and Ser42 variants of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens reveal that Arg42 is essential for NADPH binding., Eppink MH, Schreuder HA, van Berkel WJ, Eur J Biochem. 1998 Apr 1;253(1):194-201. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9578477 9578477]
[[Category: 4-hydroxybenzoate 3-monooxygenase]]
[[Category: 4-hydroxybenzoate 3-monooxygenase]]
[[Category: Pseudomonas fluorescens]]
[[Category: Pseudomonas fluorescens]]
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[[Category: oxidoreductase]]
[[Category: oxidoreductase]]


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