1bhn: Difference between revisions

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[[Image:1bhn.gif|left|200px]]<br /><applet load="1bhn" size="350" color="white" frame="true" align="right" spinBox="true"
[[Image:1bhn.gif|left|200px]]
caption="1bhn, resolution 2.4&Aring;" />
 
'''NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA'''<br />
{{Structure
|PDB= 1bhn |SIZE=350|CAPTION= <scene name='initialview01'>1bhn</scene>, resolution 2.4&Aring;
|SITE= <scene name='pdbsite=A:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>A</scene>, <scene name='pdbsite=AND F:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>AND F</scene>, <scene name='pdbsite=B:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>B</scene>, <scene name='pdbsite=C:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>C</scene>, <scene name='pdbsite=D:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>D</scene> and <scene name='pdbsite=E:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>E</scene>
|LIGAND= <scene name='pdbligand=35G:GUANOSINE-3',5'-MONOPHOSPHATE'>35G</scene>
|ACTIVITY= [http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6]
|GENE=
}}
 
'''NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA'''
 


==Overview==
==Overview==
Line 7: Line 16:


==About this Structure==
==About this Structure==
1BHN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=35G:'>35G</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Nucleoside-diphosphate_kinase Nucleoside-diphosphate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.6 2.7.4.6] Known structural/functional Sites: <scene name='pdbsite=A:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>A</scene>, <scene name='pdbsite=AND F:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>AND F</scene>, <scene name='pdbsite=B:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>B</scene>, <scene name='pdbsite=C:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>C</scene>, <scene name='pdbsite=D:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>D</scene> and <scene name='pdbsite=E:C-Gmp+And+Gdp+Are+Found+(Each+w.+Half+Occupancy)+In+The+...'>E</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BHN OCA].  
1BHN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BHN OCA].  


==Reference==
==Reference==
The three-dimensional structures of two isoforms of nucleoside diphosphate kinase from bovine retina., Ladner JE, Abdulaev NG, Kakuev DL, Tordova M, Ridge KD, Gilliland GL, Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1127-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10329774 10329774]
The three-dimensional structures of two isoforms of nucleoside diphosphate kinase from bovine retina., Ladner JE, Abdulaev NG, Kakuev DL, Tordova M, Ridge KD, Gilliland GL, Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1127-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10329774 10329774]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Nucleoside-diphosphate kinase]]
[[Category: Nucleoside-diphosphate kinase]]
Line 26: Line 35:
[[Category: phosphotransferase]]
[[Category: phosphotransferase]]


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:55:22 2008''
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:11:08 2008''

Revision as of 08:11, 20 March 2008

File:1bhn.gif


Drag the structure with the mouse to rotate
1bhn, resolution 2.4Å
Sites: A, AND F, B, C, D and E
Ligands: 35G
Activity: Nucleoside-diphosphate kinase, with EC number 2.7.4.6
Coordinates: save as pdb, mmCIF, xml



NUCLEOSIDE DIPHOSPHATE KINASE ISOFORM A FROM BOVINE RETINA


Overview

The crystal structures of two isoforms of nucleoside diphosphate kinase from bovine retina overexpressed in Escherischia coli have been determined to 2.4 A resolution. Both the isoforms, NBR-A and NBR-B, are hexameric and the fold of the monomer is in agreement with NDP-kinase structures from other biological sources. Although the polypeptide chains of the two isoforms differ by only two residues, they crystallize in different space groups. NBR-A crystallizes in space group P212121 with an entire hexamer in the asymmetric unit, while NBR-B crystallizes in space group P43212 with a trimer in the asymmetric unit. The highly conserved nucleotide-binding site observed in other nucleoside diphosphate kinase structures is also observed here. Both NBR-A and NBR-B were crystallized in the presence of cGMP. The nucleotide is bound with the base in the anti conformation. The NBR-A active site contained both cGMP and GDP each bound at half occupancy. Presumably, NBR-A had retained GDP (or GTP) from the purification process. The NBR-B active site contained only cGMP.

About this Structure

1BHN is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

The three-dimensional structures of two isoforms of nucleoside diphosphate kinase from bovine retina., Ladner JE, Abdulaev NG, Kakuev DL, Tordova M, Ridge KD, Gilliland GL, Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1127-35. PMID:10329774

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