1bos: Difference between revisions
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[[Image:1bos.gif|left|200px]] | [[Image:1bos.gif|left|200px]] | ||
'''SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR''' | {{Structure | ||
|PDB= 1bos |SIZE=350|CAPTION= <scene name='initialview01'>1bos</scene>, resolution 2.8Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1BOS is a [ | 1BOS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_h30,_bacteriophage_h19b Bacteriophage h30, bacteriophage h19b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOS OCA]. | ||
==Reference== | ==Reference== | ||
Structure of the shiga-like toxin I B-pentamer complexed with an analogue of its receptor Gb3., Ling H, Boodhoo A, Hazes B, Cummings MD, Armstrong GD, Brunton JL, Read RJ, Biochemistry. 1998 Feb 17;37(7):1777-88. PMID:[http:// | Structure of the shiga-like toxin I B-pentamer complexed with an analogue of its receptor Gb3., Ling H, Boodhoo A, Hazes B, Cummings MD, Armstrong GD, Brunton JL, Read RJ, Biochemistry. 1998 Feb 17;37(7):1777-88. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9485303 9485303] | ||
[[Category: Bacteriophage h30, bacteriophage h19b]] | [[Category: Bacteriophage h30, bacteriophage h19b]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: toxin]] | [[Category: toxin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:13:50 2008'' | ||
Revision as of 08:13, 20 March 2008
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| 1bos, resolution 2.8Å | |||||||||||||
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| Ligands: | GAL | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR
Overview
Shiga-like toxin I (SLT-I) is a virulence factor of Escherichia coli strains that cause disease in humans. Like other members of the Shiga toxin family, it consists of an enzymatic (A) subunit and five copies of a binding subunit (the B-pentamer). The B-pentamer binds to a specific glycolipid, globotriaosylceramide (Gb3), on the surface of target cells and thereby plays a crucial role in the entry of the toxin. Here we present the crystal structure at 2.8 A resolution of the SLT-I B-pentamer complexed with an analogue of the Gb3 trisaccharide. The structure reveals a surprising density of binding sites, with three trisaccharide molecules bound to each B-subunit monomer of 69 residues. All 15 trisaccharides bind to one side of the B-pentamer, providing further evidence that this side faces the cell membrane. The structural model is consistent with data from site-directed mutagenesis and binding of carbohydrate analogues, and allows the rational design of therapeutic Gb3 analogues that block the attachment of toxin to cells.
About this Structure
1BOS is a Single protein structure of sequence from Bacteriophage h30, bacteriophage h19b. Full crystallographic information is available from OCA.
Reference
Structure of the shiga-like toxin I B-pentamer complexed with an analogue of its receptor Gb3., Ling H, Boodhoo A, Hazes B, Cummings MD, Armstrong GD, Brunton JL, Read RJ, Biochemistry. 1998 Feb 17;37(7):1777-88. PMID:9485303
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