1bvs: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1bvs.gif|left|200px]] | [[Image:1bvs.gif|left|200px]] | ||
'''RUVA COMPLEXED TO A HOLLIDAY JUNCTION.''' | {{Structure | ||
|PDB= 1bvs |SIZE=350|CAPTION= <scene name='initialview01'>1bvs</scene>, resolution 3.00Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''RUVA COMPLEXED TO A HOLLIDAY JUNCTION.''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1BVS is a [ | 1BVS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_leprae Mycobacterium leprae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BVS OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of an octameric RuvA-Holliday junction complex., Roe SM, Barlow T, Brown T, Oram M, Keeley A, Tsaneva IR, Pearl LH, Mol Cell. 1998 Sep;2(3):361-72. PMID:[http:// | Crystal structure of an octameric RuvA-Holliday junction complex., Roe SM, Barlow T, Brown T, Oram M, Keeley A, Tsaneva IR, Pearl LH, Mol Cell. 1998 Sep;2(3):361-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9774974 9774974] | ||
[[Category: Mycobacterium leprae]] | [[Category: Mycobacterium leprae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 19: | Line 28: | ||
[[Category: holliday junction resolvase component]] | [[Category: holliday junction resolvase component]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:16:27 2008'' | ||
Revision as of 08:16, 20 March 2008
| |||||||||||||
| 1bvs, resolution 3.00Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
RUVA COMPLEXED TO A HOLLIDAY JUNCTION.
Overview
Holliday junctions occur as intermediates in homologous recombination and DNA repair. In bacteria, resolution of Holliday junctions is accomplished by the RuvABC system, consisting of a junction-specific helicase complex RuvAB, which promotes branch migration, and a junction-specific endonuclease RuvC, which nicks two strands. The crystal structure of a complex between the RuvA protein of M. leprae and a synthetic four-way junction has now been determined. Rather than binding on the open surface of a RuvA tetramer as previously suggested, the DNA is sandwiched between two RuvA tetramers, which form a closed octameric shell, stabilized by a conserved tetramer-tetramer interface. Interactions between the DNA backbone and helix-hairpin-helix motifs from both tetramers suggest a mechanism for strand separation promoted by RuvA.
About this Structure
1BVS is a Single protein structure of sequence from Mycobacterium leprae. Full crystallographic information is available from OCA.
Reference
Crystal structure of an octameric RuvA-Holliday junction complex., Roe SM, Barlow T, Brown T, Oram M, Keeley A, Tsaneva IR, Pearl LH, Mol Cell. 1998 Sep;2(3):361-72. PMID:9774974
Page seeded by OCA on Thu Mar 20 10:16:27 2008
