1bw6: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1bw6.gif|left|200px]] | [[Image:1bw6.gif|left|200px]] | ||
'''HUMAN CENTROMERE PROTEIN B (CENP-B) DNA BINDIGN DOMAIN RP1''' | {{Structure | ||
|PDB= 1bw6 |SIZE=350|CAPTION= <scene name='initialview01'>1bw6</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''HUMAN CENTROMERE PROTEIN B (CENP-B) DNA BINDIGN DOMAIN RP1''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1BW6 is a [ | 1BW6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BW6 OCA]. | ||
==Reference== | ==Reference== | ||
A helix-turn-helix structure unit in human centromere protein B (CENP-B)., Iwahara J, Kigawa T, Kitagawa K, Masumoto H, Okazaki T, Yokoyama S, EMBO J. 1998 Feb 2;17(3):827-37. PMID:[http:// | A helix-turn-helix structure unit in human centromere protein B (CENP-B)., Iwahara J, Kigawa T, Kitagawa K, Masumoto H, Okazaki T, Yokoyama S, EMBO J. 1998 Feb 2;17(3):827-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9451007 9451007] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 25: | Line 34: | ||
[[Category: riken structural genomics/proteomics initiative]] | [[Category: riken structural genomics/proteomics initiative]] | ||
[[Category: rsgi]] | [[Category: rsgi]] | ||
[[Category: structural | [[Category: structural genomic]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:16:31 2008'' | ||
Revision as of 08:16, 20 March 2008
| |||||||||||||
| 1bw6 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
HUMAN CENTROMERE PROTEIN B (CENP-B) DNA BINDIGN DOMAIN RP1
Overview
CENP-B has been suggested to organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. The N-terminal portion of CENP-B is a 15 kDa DNA binding domain (DBD) consisting of two repeating units, RP1 and RP2. The DBD specifically binds to the CENP-B box sequence (17 bp) in centromere DNA. We determined the solution structure of human CENP-B DBD RP1 by multi-dimensional 1H, 13C and 15N NMR methods. The CENP-B DBD RP1 structure consists of four helices and has a helix-turn-helix structure. The overall folding is similar to those of some other eukaryotic DBDs, although significant sequence homology with these proteins was not found. The DBD of yeast RAP1, a telomere binding protein, is most similar to CENP-B DBD RP1. We studied the interaction between CENP-B DBD RP1 and the CENP-B box by the use of NMR chemical shift perturbation. The results suggest that CENP-B DBD RP1 interacts with one of the essential regions of the CENP-B box DNA, mainly at the N-terminal basic region, the N-terminal portion of helix 2 and helix 3.
About this Structure
1BW6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
A helix-turn-helix structure unit in human centromere protein B (CENP-B)., Iwahara J, Kigawa T, Kitagawa K, Masumoto H, Okazaki T, Yokoyama S, EMBO J. 1998 Feb 2;17(3):827-37. PMID:9451007
Page seeded by OCA on Thu Mar 20 10:16:31 2008
