1c4l: Difference between revisions
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[[Image:1c4l.gif|left|200px]] | [[Image:1c4l.gif|left|200px]] | ||
'''SOLUTION STRUCTURE OF AN RNA DUPLEX INCLUDING A C-U BASE-PAIR''' | {{Structure | ||
|PDB= 1c4l |SIZE=350|CAPTION= <scene name='initialview01'>1c4l</scene> | |||
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|LIGAND= | |||
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'''SOLUTION STRUCTURE OF AN RNA DUPLEX INCLUDING A C-U BASE-PAIR''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1C4L is a [ | 1C4L is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C4L OCA]. | ||
==Reference== | ==Reference== | ||
Solution structure of an RNA duplex including a C-U base pair., Tanaka Y, Kojima C, Yamazaki T, Kodama TS, Yasuno K, Miyashita S, Ono A, Ono A, Kainosho M, Kyogoku Y, Biochemistry. 2000 Jun 20;39(24):7074-80. PMID:[http:// | Solution structure of an RNA duplex including a C-U base pair., Tanaka Y, Kojima C, Yamazaki T, Kodama TS, Yasuno K, Miyashita S, Ono A, Ono A, Kainosho M, Kyogoku Y, Biochemistry. 2000 Jun 20;39(24):7074-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10852704 10852704] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Kainosho, M.]] | [[Category: Kainosho, M.]] | ||
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[[Category: c-u base pair]] | [[Category: c-u base pair]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:19:46 2008'' | ||
Revision as of 08:19, 20 March 2008
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SOLUTION STRUCTURE OF AN RNA DUPLEX INCLUDING A C-U BASE-PAIR
Overview
The formation of the C-U base pair in a duplex was observed in solution by means of the temperature profile of (15)N chemical shifts, and the precise geometry of the C-U base pair was also determined by NOE-based structure calculation. From the solution structure of the RNA oligomer, r[CGACUCAGG].r[CCUGCGUCG], it was found that a single C-U mismatch preferred being stacked in the duplex rather than being flipped-out even in solution. Moreover, it adopts an irregular geometry, where the amino nitrogen (N4) of the cytidine and keto-oxygen (O4) of the uridine are within hydrogen-bonding distance, as seen in crystals. To further prove the presence of a hydrogen bond in the C-U pair, we employed a point-labeled cytidine at the exocyclic amino nitrogen of the cytidine in the C-U pair. The temperature profile of its (15)N chemical shift showed a sigmoidal transition curve, indicating the presence of a hydrogen bond in the C-U pair in the duplex.
About this Structure
1C4L is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of an RNA duplex including a C-U base pair., Tanaka Y, Kojima C, Yamazaki T, Kodama TS, Yasuno K, Miyashita S, Ono A, Ono A, Kainosho M, Kyogoku Y, Biochemistry. 2000 Jun 20;39(24):7074-80. PMID:10852704
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