1c8c: Difference between revisions
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[[Image:1c8c.gif|left|200px]] | [[Image:1c8c.gif|left|200px]] | ||
'''CRYSTAL STRUCTURES OF THE CHROMOSOMAL PROTEINS SSO7D/SAC7D BOUND TO DNA CONTAINING T-G MISMATCHED BASE PAIRS''' | {{Structure | ||
|PDB= 1c8c |SIZE=350|CAPTION= <scene name='initialview01'>1c8c</scene>, resolution 1.45Å | |||
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|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
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'''CRYSTAL STRUCTURES OF THE CHROMOSOMAL PROTEINS SSO7D/SAC7D BOUND TO DNA CONTAINING T-G MISMATCHED BASE PAIRS''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1C8C is a [ | 1C8C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C8C OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs., Su S, Gao YG, Robinson H, Liaw YC, Edmondson SP, Shriver JW, Wang AH, J Mol Biol. 2000 Oct 27;303(3):395-403. PMID:[http:// | Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs., Su S, Gao YG, Robinson H, Liaw YC, Edmondson SP, Shriver JW, Wang AH, J Mol Biol. 2000 Oct 27;303(3):395-403. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11031116 11031116] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sulfolobus solfataricus]] | [[Category: Sulfolobus solfataricus]] | ||
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[[Category: achaeabacteria]] | [[Category: achaeabacteria]] | ||
[[Category: dna binding protein]] | [[Category: dna binding protein]] | ||
[[Category: | [[Category: electrostatic]] | ||
[[Category: hyperthermophile]] | [[Category: hyperthermophile]] | ||
[[Category: molecular modeling]] | [[Category: molecular modeling]] | ||
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[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:21:08 2008'' | ||
Revision as of 08:21, 20 March 2008
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| 1c8c, resolution 1.45Å | |||||||||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
CRYSTAL STRUCTURES OF THE CHROMOSOMAL PROTEINS SSO7D/SAC7D BOUND TO DNA CONTAINING T-G MISMATCHED BASE PAIRS
Overview
Sso7d and Sac7d are two small chromatin proteins from the hyperthermophilic archaeabacterium Sulfolobus solfataricus and Sulfolobus acidocaldarius, respectively. The crystal structures of Sso7d-GTGATCGC, Sac7d-GTGATCGC and Sac7d-GTGATCAC have been determined and refined at 1.45 A, 2.2 A and 2.2 A, respectively, to investigate the DNA binding property of Sso7d/Sac7d in the presence of a T-G mismatch base-pair. Detailed structural analysis revealed that the intercalation site includes the T-G mismatch base-pair and Sso7d/Sac7d bind to that mismatch base-pair in a manner similar to regular DNA. In the Sso7d-GTGATCGC complex, a new inter-strand hydrogen bond between T2O4 and C14N4 is formed and well-order bridging water molecules are found. The results suggest that the less stable DNA stacking site involving a T-G mismatch may be a preferred site for protein side-chain intercalation.
About this Structure
1C8C is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
Reference
Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs., Su S, Gao YG, Robinson H, Liaw YC, Edmondson SP, Shriver JW, Wang AH, J Mol Biol. 2000 Oct 27;303(3):395-403. PMID:11031116
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