1cgt: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1cgt.gif|left|200px]] | [[Image:1cgt.gif|left|200px]] | ||
'''STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE REFINED AT 2.0 ANGSTROMS RESOLUTION''' | {{Structure | ||
|PDB= 1cgt |SIZE=350|CAPTION= <scene name='initialview01'>1cgt</scene>, resolution 2.0Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] | |||
|GENE= | |||
}} | |||
'''STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE REFINED AT 2.0 ANGSTROMS RESOLUTION''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1CGT is a [ | 1CGT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGT OCA]. | ||
==Reference== | ==Reference== | ||
Structure of cyclodextrin glycosyltransferase refined at 2.0 A resolution., Klein C, Schulz GE, J Mol Biol. 1991 Feb 20;217(4):737-50. PMID:[http:// | Structure of cyclodextrin glycosyltransferase refined at 2.0 A resolution., Klein C, Schulz GE, J Mol Biol. 1991 Feb 20;217(4):737-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1826034 1826034] | ||
[[Category: Bacillus circulans]] | [[Category: Bacillus circulans]] | ||
[[Category: Cyclomaltodextrin glucanotransferase]] | [[Category: Cyclomaltodextrin glucanotransferase]] | ||
| Line 19: | Line 28: | ||
[[Category: glycosyltransferase]] | [[Category: glycosyltransferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:24:11 2008'' | ||
Revision as of 08:24, 20 March 2008
| |||||||||||||
| 1cgt, resolution 2.0Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | CA | ||||||||||||
| Activity: | Cyclomaltodextrin glucanotransferase, with EC number 2.4.1.19 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE REFINED AT 2.0 ANGSTROMS RESOLUTION
Overview
The previously reported structural model of cyclodextrin glycosyltransferase (EC 2.4.1.19) from Bacillus circulans has been improved. For this purpose the known sequence was built into an electron density map established by multiple isomorphous replacement and subsequent solvent-flattening at 2.5 A resolution. The resulting model was refined at 2.0 A resolution using a simulated annealing refinement method. Based on 70,171 independent reflections in the range 7.0 to 2.0 A resolution, a final R-factor of 17.6% was obtained with a model obeying standard geometry within 0.013 A in bond lengths and 2.7 degrees in bond angles. The final model consists of all 684 amino acid residues, two calcium ions and 588 solvent molecules.
About this Structure
1CGT is a Single protein structure of sequence from Bacillus circulans. Full crystallographic information is available from OCA.
Reference
Structure of cyclodextrin glycosyltransferase refined at 2.0 A resolution., Klein C, Schulz GE, J Mol Biol. 1991 Feb 20;217(4):737-50. PMID:1826034
Page seeded by OCA on Thu Mar 20 10:24:11 2008
