1cz0: Difference between revisions
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[[Image:1cz0.gif|left|200px]] | [[Image:1cz0.gif|left|200px]] | ||
'''INTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL ION''' | {{Structure | ||
|PDB= 1cz0 |SIZE=350|CAPTION= <scene name='initialview01'>1cz0</scene>, resolution 2.1Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''INTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL ION''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1CZ0 is a [ | 1CZ0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CZ0 OCA]. | ||
==Reference== | ==Reference== | ||
A novel endonuclease mechanism directly visualized for I-PpoI., Galburt EA, Chevalier B, Tang W, Jurica MS, Flick KE, Monnat RJ Jr, Stoddard BL, Nat Struct Biol. 1999 Dec;6(12):1096-9. PMID:[http:// | A novel endonuclease mechanism directly visualized for I-PpoI., Galburt EA, Chevalier B, Tang W, Jurica MS, Flick KE, Monnat RJ Jr, Stoddard BL, Nat Struct Biol. 1999 Dec;6(12):1096-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10581547 10581547] | ||
[[Category: Physarum polycephalum]] | [[Category: Physarum polycephalum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:30:36 2008'' | ||
Revision as of 08:30, 20 March 2008
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| 1cz0, resolution 2.1Å | |||||||||||||
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| Ligands: | ZN and NA | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
INTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL ION
Overview
A novel mechanism of DNA endonucleolytic cleavage has been visualized for the homing endonuclease I-PpoI by trapping the uncleaved enzyme-substrate complex and comparing it to the previously visualized product complex. This enzyme employs a unique single metal mechanism. A magnesium ion is coordinated by an asparagine residue and two DNA oxygen atoms and stabilizes the phosphoanion transition state and the 3'oxygen leaving group. A hydrolytic water molecule is activated by a histidine residue for an in-line attack on the scissile phosphate. A strained enzyme-substrate-metal complex is formed before cleavage, then relaxed during the reaction.
About this Structure
1CZ0 is a Single protein structure of sequence from Physarum polycephalum. Full crystallographic information is available from OCA.
Reference
A novel endonuclease mechanism directly visualized for I-PpoI., Galburt EA, Chevalier B, Tang W, Jurica MS, Flick KE, Monnat RJ Jr, Stoddard BL, Nat Struct Biol. 1999 Dec;6(12):1096-9. PMID:10581547
Page seeded by OCA on Thu Mar 20 10:30:36 2008
