1d5t: Difference between revisions
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'''GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR, ALPHA-ISOFORM''' | {{Structure | ||
|PDB= 1d5t |SIZE=350|CAPTION= <scene name='initialview01'>1d5t</scene>, resolution 1.04Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR, ALPHA-ISOFORM''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1D5T is a [ | 1D5T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D5T OCA]. | ||
==Reference== | ==Reference== | ||
A new functional domain of guanine nucleotide dissociation inhibitor (alpha-GDI) involved in Rab recycling., Luan P, Heine A, Zeng K, Moyer B, Greasely SE, Kuhn P, Balch WE, Wilson IA, Traffic. 2000 Mar;1(3):270-81. PMID:[http:// | A new functional domain of guanine nucleotide dissociation inhibitor (alpha-GDI) involved in Rab recycling., Luan P, Heine A, Zeng K, Moyer B, Greasely SE, Kuhn P, Balch WE, Wilson IA, Traffic. 2000 Mar;1(3):270-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11208110 11208110] | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: ultra-high resolution]] | [[Category: ultra-high resolution]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:33:19 2008'' | ||
Revision as of 08:33, 20 March 2008
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| 1d5t, resolution 1.04Å | |||||||||||||
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| Ligands: | SO4 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR, ALPHA-ISOFORM
Overview
Guanine nucleotide dissociation inhibitor (GDI) is a 55-kDa protein that functions in vesicular membrane transport to recycle Rab GTPases. We have now determined the crystal structure of bovine alpha-GDI at ultra-high resolution (1.04 A). Refinement at this resolution highlighted a region with high mobility of its main-chain residues. This corresponded to a surface loop in the primarily alpha-helical domain II at the base of alpha-GDI containing the previously uncharacterized sequence-conserved region (SCR) 3A. Site-directed mutagenesis showed that this mobile loop plays a crucial role in binding of GDI to membranes and extraction of membrane-bound Rab. This domain, referred to as the mobile effector loop, in combination with Rab-binding residues found in the multi-sheet domain I at the apex of alpha-GDI may provide flexibility for recycling of diverse Rab GTPases. We propose that conserved residues in domains I and II synergize to form the functional face of GDI, and that domain II mediates a critical step in Rab recycling during vesicle fusion.
About this Structure
1D5T is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
A new functional domain of guanine nucleotide dissociation inhibitor (alpha-GDI) involved in Rab recycling., Luan P, Heine A, Zeng K, Moyer B, Greasely SE, Kuhn P, Balch WE, Wilson IA, Traffic. 2000 Mar;1(3):270-81. PMID:11208110
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