1ddp: Difference between revisions
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[[Image:1ddp.gif|left|200px]] | [[Image:1ddp.gif|left|200px]] | ||
'''SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [CATAGCTATG]2 INTERSTRAND CROSS-LINK''' | {{Structure | ||
|PDB= 1ddp |SIZE=350|CAPTION= <scene name='initialview01'>1ddp</scene> | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CPT:CIS-PLATINUM-(NH3)2'>CPT</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [CATAGCTATG]2 INTERSTRAND CROSS-LINK''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1DDP is a [ | 1DDP is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DDP OCA]. | ||
==Reference== | ==Reference== | ||
Solution structure of a cisplatin-induced DNA interstrand cross-link., Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB, Science. 1995 Dec 15;270(5243):1842-5. PMID:[http:// | Solution structure of a cisplatin-induced DNA interstrand cross-link., Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB, Science. 1995 Dec 15;270(5243):1842-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8525382 8525382] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Drobny, G P.]] | [[Category: Drobny, G P.]] | ||
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[[Category: nmr]] | [[Category: nmr]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:36:50 2008'' | ||
Revision as of 08:36, 20 March 2008
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| Ligands: | CPT | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
SOLUTION STRUCTURE OF A CISPLATIN-INDUCED [CATAGCTATG]2 INTERSTRAND CROSS-LINK
Overview
The widely used antitumor drug cis-diamminedichloroplatinum(II) (cisplatin or cis-DDP) reacts with DNA, cross-linking two purine residues through the N7 atoms, which reside in the major groove in B-form DNA. The solution structure of the short duplex [d(CAT-AGCTATG)]2 cross-linked at the GC:GC site was determined by nuclear magnetic resonance (NMR). The deoxyguanosine-bridging cis-diammineplatinum(II) lies in the minor groove, and the complementary deoxycytidines are extrahelical. The double helix is locally reversed to a left-handed form, and the helix is unwound and bent toward the minor groove. These findings were independently confirmed by results from a phase-sensitive gel electrophoresis bending assay. The NMR structure differs markedly from previously proposed models but accounts for the chemical reactivity, the unwinding, and the bending of cis-DDP interstrand cross-linked DNA and may be important in the formation and repair of these cross-links in chromatin.
About this Structure
1DDP is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of a cisplatin-induced DNA interstrand cross-link., Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB, Science. 1995 Dec 15;270(5243):1842-5. PMID:8525382
Page seeded by OCA on Thu Mar 20 10:36:50 2008
