1f0v: Difference between revisions
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[[Image:1f0v.gif|left|200px]] | [[Image:1f0v.gif|left|200px]] | ||
'''Crystal structure of an Rnase A dimer displaying a new type of 3D domain swapping''' | {{Structure | ||
|PDB= 1f0v |SIZE=350|CAPTION= <scene name='initialview01'>1f0v</scene>, resolution 1.7Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] | |||
|GENE= | |||
}} | |||
'''Crystal structure of an Rnase A dimer displaying a new type of 3D domain swapping''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1F0V is a [ | 1F0V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F0V OCA]. | ||
==Reference== | ==Reference== | ||
A domain-swapped RNase A dimer with implications for amyloid formation., Liu Y, Gotte G, Libonati M, Eisenberg D, Nat Struct Biol. 2001 Mar;8(3):211-4. PMID:[http:// | A domain-swapped RNase A dimer with implications for amyloid formation., Liu Y, Gotte G, Libonati M, Eisenberg D, Nat Struct Biol. 2001 Mar;8(3):211-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11224563 11224563] | ||
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Pancreatic ribonuclease]] | [[Category: Pancreatic ribonuclease]] | ||
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[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: PO4]] | [[Category: PO4]] | ||
[[Category: bovine | [[Category: bovine pancrea]] | ||
[[Category: crystal]] | [[Category: crystal]] | ||
[[Category: domain swapping]] | [[Category: domain swapping]] | ||
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[[Category: x-ray]] | [[Category: x-ray]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:02:53 2008'' | ||
Revision as of 09:02, 20 March 2008
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| 1f0v, resolution 1.7Å | |||||||||||||
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| Ligands: | PO4 and GOL | ||||||||||||
| Activity: | Pancreatic ribonuclease, with EC number 3.1.27.5 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of an Rnase A dimer displaying a new type of 3D domain swapping
Overview
Bovine pancreatic ribonuclease (RNase A) forms two types of dimers (a major and a minor component) upon concentration in mild acid. These two dimers exhibit different biophysical and biochemical properties. Earlier we reported that the minor dimer forms by swapping its N-terminal alpha-helix with that of an identical molecule. Here we find that the major dimer forms by swapping its C-terminal beta-strand, thus revealing the first example of three-dimensional (3D) domain swapping taking place in different parts of the same protein. This feature permits RNase A to form tightly bonded higher oligomers. The hinge loop of the major dimer, connecting the swapped beta-strand to the protein core, resembles a short segment of the polar zipper proposed by Perutz and suggests a model for aggregate formation by 3D domain swapping with a polar zipper.
About this Structure
1F0V is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
A domain-swapped RNase A dimer with implications for amyloid formation., Liu Y, Gotte G, Libonati M, Eisenberg D, Nat Struct Biol. 2001 Mar;8(3):211-4. PMID:11224563
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