1f4n: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1f4n.jpg|left|200px]] | [[Image:1f4n.jpg|left|200px]] | ||
'''C2 CRYSTAL STRUCTURE OF ALA2ILE2-6, A VERSION OF ROP WITH A REPACKED HYDROPHOBIC CORE AND A NEW FOLD.''' | {{Structure | ||
|PDB= 1f4n |SIZE=350|CAPTION= <scene name='initialview01'>1f4n</scene>, resolution 1.90Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''C2 CRYSTAL STRUCTURE OF ALA2ILE2-6, A VERSION OF ROP WITH A REPACKED HYDROPHOBIC CORE AND A NEW FOLD.''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1F4N is a [ | 1F4N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F4N OCA]. | ||
==Reference== | ==Reference== | ||
Dramatic structural and thermodynamic consequences of repacking a protein's hydrophobic core., Willis MA, Bishop B, Regan L, Brunger AT, Structure. 2000 Dec 15;8(12):1319-28. PMID:[http:// | Dramatic structural and thermodynamic consequences of repacking a protein's hydrophobic core., Willis MA, Bishop B, Regan L, Brunger AT, Structure. 2000 Dec 15;8(12):1319-28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11188696 11188696] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 27: | Line 36: | ||
[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:04:21 2008'' | ||
Revision as of 09:04, 20 March 2008
| |||||||||||||
| 1f4n, resolution 1.90Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | CA and MPD | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
C2 CRYSTAL STRUCTURE OF ALA2ILE2-6, A VERSION OF ROP WITH A REPACKED HYDROPHOBIC CORE AND A NEW FOLD.
Overview
BACKGROUND: Rop is an RNA binding, dimeric, four-helix bundle protein with a well-defined, regular hydrophobic core ideally suited for redesign studies. A family of Rop variants in which the hydrophobic core was systematically redesigned has previously been created and characterized. RESULTS: We present a structural and thermodynamic analysis of Ala2Ile2-6, a variant of Rop with an extensively redesigned hydrophobic core. The structure of Ala2Ile2-6 reveals a completely new fold formed by a conformational "flip" of the two protomers around the dimeric interface. The free-energy profile of Ala2Ile2-6 is also very different from that of wild-type Rop. Ala2Ile2-6 has a higher melting temperature than Rop, but undergoes a slightly smaller free-energy change on unfolding. CONCLUSIONS: The structure of Ala2Ile2-6, along with molecular modeling results, demonstrate the importance of tight packing of core residues and the adoption of favorable core side chain rotamer values in determining helix-helix interactions in the four-helix bundle fold. Structural disorder at the N and C termini of Ala2Ile2-6 provides a basis for the large differences in the enthalpy and entropy of Ala2Ile2-6 folding compared with wildtype Rop.
About this Structure
1F4N is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Dramatic structural and thermodynamic consequences of repacking a protein's hydrophobic core., Willis MA, Bishop B, Regan L, Brunger AT, Structure. 2000 Dec 15;8(12):1319-28. PMID:11188696
Page seeded by OCA on Thu Mar 20 11:04:21 2008
