1f5u: Difference between revisions
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[[Image:1f5u.gif|left|200px]] | [[Image:1f5u.gif|left|200px]] | ||
'''SOLUTION STRUCTURE OF THE KISSING DIMER OF H3 GACG STEM-LOOP IN THE 5'-END DIMERIZATION SIGNAL OF MOLONEY MURINE LEUKEMIA VIRUS GENOMIC RNA''' | {{Structure | ||
|PDB= 1f5u |SIZE=350|CAPTION= <scene name='initialview01'>1f5u</scene> | |||
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'''SOLUTION STRUCTURE OF THE KISSING DIMER OF H3 GACG STEM-LOOP IN THE 5'-END DIMERIZATION SIGNAL OF MOLONEY MURINE LEUKEMIA VIRUS GENOMIC RNA''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1F5U is a [ | 1F5U is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F5U OCA]. | ||
==Reference== | ==Reference== | ||
A retroviral RNA kissing complex containing only two G.C base pairs., Kim CH, Tinoco I Jr, Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9396-401. PMID:[http:// | A retroviral RNA kissing complex containing only two G.C base pairs., Kim CH, Tinoco I Jr, Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9396-401. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10931958 10931958] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Jr., I Tinoco.]] | [[Category: Jr., I Tinoco.]] | ||
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[[Category: tetraloop]] | [[Category: tetraloop]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:04:50 2008'' | ||
Revision as of 09:04, 20 March 2008
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SOLUTION STRUCTURE OF THE KISSING DIMER OF H3 GACG STEM-LOOP IN THE 5'-END DIMERIZATION SIGNAL OF MOLONEY MURINE LEUKEMIA VIRUS GENOMIC RNA
Overview
The dimerization of viral RNA through noncovalent interactions at their 5' ends is a key step in the life cycle of retroviruses. In Moloney murine leukemia virus, three stem-loops are important in this process. One is a self-complementary tetraloop (H1), but the other two stem-loops (H2, H3) contain highly conserved GACG tetraloops that are not self-complementary sequences. Using two-dimensional NMR, we determined the structure of the H3 stem-loop. Surprisingly, it forms a stable, homodimeric kissing complex through only two intermolecular G small middle dotC base pairs. Cross-strand interactions of the adenines adjacent to the intermolecular G small middle dotC base pairs, plus unusual strong electrostatic interactions around the base pairs, contribute to the unexpected stability. This structure shows how even stem-loops without self-complementary sequences can facilitate the intermolecular recognition between two identical RNAs, and thus initiate dimerization and encapsidation of retroviral RNAs.
About this Structure
1F5U is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A retroviral RNA kissing complex containing only two G.C base pairs., Kim CH, Tinoco I Jr, Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9396-401. PMID:10931958
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