1fml: Difference between revisions
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[[Image:1fml.gif|left|200px]] | [[Image:1fml.gif|left|200px]] | ||
'''CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP''' | {{Structure | ||
|PDB= 1fml |SIZE=350|CAPTION= <scene name='initialview01'>1fml</scene>, resolution 2.75Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=A3P:ADENOSINE-3'-5'-DIPHOSPHATE'>A3P</scene> and <scene name='pdbligand=RTL:RETINOL'>RTL</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1FML is a [ | 1FML is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FML OCA]. | ||
==Reference== | ==Reference== | ||
A helical lid converts a sulfotransferase to a dehydratase., Pakhomova S, Kobayashi M, Buck J, Newcomer ME, Nat Struct Biol. 2001 May;8(5):447-51. PMID:[http:// | A helical lid converts a sulfotransferase to a dehydratase., Pakhomova S, Kobayashi M, Buck J, Newcomer ME, Nat Struct Biol. 2001 May;8(5):447-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11323722 11323722] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Spodoptera frugiperda]] | [[Category: Spodoptera frugiperda]] | ||
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[[Category: RTL]] | [[Category: RTL]] | ||
[[Category: 5'-diphosphate]] | [[Category: 5'-diphosphate]] | ||
[[Category: adenosine | [[Category: adenosine 3s']] | ||
[[Category: dehydratase]] | [[Category: dehydratase]] | ||
[[Category: retinol]] | [[Category: retinol]] | ||
[[Category: sulfotransferase]] | [[Category: sulfotransferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:11:08 2008'' | ||
Revision as of 09:11, 20 March 2008
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| 1fml, resolution 2.75Å | |||||||||||||
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| Ligands: | CA, A3P and RTL | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
CRYSTAL STRUCTURE OF RETINOL DEHYDRATASE IN A COMPLEX WITH RETINOL AND PAP
Overview
We report here the crystal structure of retinol dehydratase, an enzyme that catalyzes the synthesis of anhydroretinol. The enzyme is a member of the sulfotransferase superfamily and its crystal structure reveals the insertion of a helical lid into a canonical sulfotransferase fold. Site-directed mutations demonstrate that this inserted lid is necessary for anhydroretinol production but not for sulfonation; thus, insertion of a helical lid can convert a sulfotransferase into a dehydratase.
About this Structure
1FML is a Single protein structure of sequence from Spodoptera frugiperda. Full crystallographic information is available from OCA.
Reference
A helical lid converts a sulfotransferase to a dehydratase., Pakhomova S, Kobayashi M, Buck J, Newcomer ME, Nat Struct Biol. 2001 May;8(5):447-51. PMID:11323722
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