1gid: Difference between revisions
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[[Image:1gid.jpg|left|200px]] | [[Image:1gid.jpg|left|200px]] | ||
'''CRYSTAL STRUCTURE OF A GROUP I RIBOZYME DOMAIN: PRINCIPLES OF RNA PACKING''' | {{Structure | ||
|PDB= 1gid |SIZE=350|CAPTION= <scene name='initialview01'>1gid</scene>, resolution 2.500Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=NCO:COBALT HEXAMMINE ION'>NCO</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF A GROUP I RIBOZYME DOMAIN: PRINCIPLES OF RNA PACKING''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1GID is a [ | 1GID is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GID OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of a group I ribozyme domain: principles of RNA packing., Cate JH, Gooding AR, Podell E, Zhou K, Golden BL, Kundrot CE, Cech TR, Doudna JA, Science. 1996 Sep 20;273(5282):1678-85. PMID:[http:// | Crystal structure of a group I ribozyme domain: principles of RNA packing., Cate JH, Gooding AR, Podell E, Zhou K, Golden BL, Kundrot CE, Cech TR, Doudna JA, Science. 1996 Sep 20;273(5282):1678-85. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8781224 8781224] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Cate, J H.]] | [[Category: Cate, J H.]] | ||
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[[Category: rna]] | [[Category: rna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:23:30 2008'' | ||
Revision as of 09:23, 20 March 2008
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| 1gid, resolution 2.500Å | |||||||||||||
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| Ligands: | MG and NCO | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
CRYSTAL STRUCTURE OF A GROUP I RIBOZYME DOMAIN: PRINCIPLES OF RNA PACKING
Overview
Group I self-splicing introns catalyze their own excision from precursor RNAs by way of a two-step transesterification reaction. The catalytic core of these ribozymes is formed by two structural domains. The 2.8-angstrom crystal structure of one of these, the P4-P6 domain of the Tetrahymena thermophila intron, is described. In the 160-nucleotide domain, a sharp bend allows stacked helices of the conserved core to pack alongside helices of an adjacent region. Two specific long-range interactions clamp the two halves of the domain together: a two-Mg2+-coordinated adenosine-rich corkscrew plugs into the minor groove of a helix, and a GAAA hairpin loop binds to a conserved 11-nucleotide internal loop. Metal- and ribose-mediated backbone contacts further stabilize the close side-by-side helical packing. The structure indicates the extent of RNA packing required for the function of large ribozymes, the spliceosome, and the ribosome.
About this Structure
1GID is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of a group I ribozyme domain: principles of RNA packing., Cate JH, Gooding AR, Podell E, Zhou K, Golden BL, Kundrot CE, Cech TR, Doudna JA, Science. 1996 Sep 20;273(5282):1678-85. PMID:8781224
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