1hbx: Difference between revisions
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[[Image:1hbx.gif|left|200px]] | [[Image:1hbx.gif|left|200px]] | ||
'''TERNARY COMPLEX OF SAP-1 AND SRF WITH SPECIFIC SRE DNA''' | {{Structure | ||
|PDB= 1hbx |SIZE=350|CAPTION= <scene name='initialview01'>1hbx</scene>, resolution 3.15Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= SRF (132-223) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |||
}} | |||
'''TERNARY COMPLEX OF SAP-1 AND SRF WITH SPECIFIC SRE DNA''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1HBX is a [ | 1HBX is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HBX OCA]. | ||
==Reference== | ==Reference== | ||
The B-box dominates SAP-1-SRF interactions in the structure of the ternary complex., Hassler M, Richmond TJ, EMBO J. 2001 Jun 15;20(12):3018-28. PMID:[http:// | The B-box dominates SAP-1-SRF interactions in the structure of the ternary complex., Hassler M, Richmond TJ, EMBO J. 2001 Jun 15;20(12):3018-28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11406578 11406578] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: transcription complex]] | [[Category: transcription complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:35:00 2008'' | ||
Revision as of 09:35, 20 March 2008
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| 1hbx, resolution 3.15Å | |||||||||||||
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| Gene: | SRF (132-223) (Homo sapiens) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
TERNARY COMPLEX OF SAP-1 AND SRF WITH SPECIFIC SRE DNA
Overview
The serum response element (SRE) is found in several immediate-early gene promoters. This DNA sequence is necessary and sufficient for rapid transcriptional induction of the human c-fos proto-oncogene in response to stimuli external to the cell. Full activation of the SRE requires the cooperative binding of a ternary complex factor (TCF) and serum response factor (SRF) to their specific DNA sites. The X-ray structure of the human SAP-1-SRF-SRE DNA ternary complex was determined (Protein Data Bank code 1hbx). It shows SAP-1 TCF bound to SRF through interactions between the SAP-1 B-box and SRF MADS domain in addition to contacts between their respective DNA-binding motifs. The SAP-1 B-box is part of a flexible linker of which 21 amino acids become ordered upon ternary complex formation. Comparison with a similar region from the yeast MATalpha2-MCM1-DNA complex suggests a common binding motif through which MADS-box proteins may interact with additional factors such as Fli-1.
Disease
Known disease associated with this structure: Osteoarthritis of hip, female-specific, susceptibility to OMIM:[605083]
About this Structure
1HBX is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The B-box dominates SAP-1-SRF interactions in the structure of the ternary complex., Hassler M, Richmond TJ, EMBO J. 2001 Jun 15;20(12):3018-28. PMID:11406578
Page seeded by OCA on Thu Mar 20 11:35:00 2008
